Why does Clostridium acetireducens not use interspecies hydrogen transfer for growth on leucine?

Laurence Girbal, Jóhann Örlygsson, Bert J. Reinders, Jan C. Gottschal

Rannsóknarafurð: Framlag til fræðitímaritsGreinritrýni

12 Tilvitnanir (Scopus)

Útdráttur

Clostridium acetireducens is the first reported anaerobic bacterium that is dependent on acetate as an electron acceptor for growth on branched-chain amino acids and alanine. The fermentation pathway of leucine and its deamination product α-ketoisocaproate were studied in this organism. Addition of Methanobacterium formicicum to pure cultures of C. acetireducens stimulated the degradation of α-ketoisocaproate but not the degradation of leucine, indicating that the electrons produced during the oxidative deamination of leucine were not transferred to hydrogen. This conclusion is supported by the observed low NAD(P)H ferredoxin reductase activity. Not only acetate but also crotonate proved to be an appropriate electron sink for the regeneration of NAD(P)+ in this bacterium. Interestingly, C. acetireducens was shown to form polyhydroxybutyrate during growth on leucine plus acetate.
Upprunalegt tungumálEnska
Síður (frá-til)155-160
Síðufjöldi6
FræðitímaritCurrent Microbiology
Bindi35
Númer tölublaðs3
DOI
ÚtgáfustaðaÚtgefið - sep. 1997

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