Transcription factor Tfec contributes to the IL-4-inducible expression of a small group of genes in mouse macrophages including the granulocyte colony-stimulating factor receptor

Michael Rehli, Sabine Sulzbacher, Sabine Pape, Timothy Ravasi, Christine A. Wells, Sven Heinz, Liane Söllner, Carol El Chartouni, Stefan W. Krause, Eirikur Steingrimsson, David A. Hume, Reinhard Andreesen

Rannsóknarafurð: Framlag til fræðitímaritsGreinritrýni

53 Tilvitnanir (Scopus)

Útdráttur

Expression of the mouse transcription factor EC (Tfec) is restricted to the myeloid compartment, suggesting a function for Tfec in the development or function of these cells. However, mice lacking Tfec develop normally, indicating a redundant role for Tfec in myeloid cell development. We now report that Tfec is specifically induced in bone marrow-derived macrophages upon stimulation with the Th2 cytokines, IL-4 and IL-13, or LPS. LPS induced a rapid and transient up-regulation of Tfec mRNA expression and promoter activity, which was dependent on a functional NF-κB site. IL-4, however, induced a rapid, but long-lasting, increase in Tfec mRNA, which, in contrast to LPS stimulation, also resulted in detectable levels of Tfec protein. IL-4-induced transcription of Tfec was absent in macrophages lacking Stat6, and its promoter depended on two functional Stato-binding sites. A global comparison of IL-4-induced genes in both wild-type and Tfec mutant macrophages revealed a surprisingly mild phenotype with only a few genes affected by Tfec deficiency. These included the G-CSFR (Csf3r) gene that was strongly up-regulated by IL-4 in wild-type macrophages and, to a lesser extent, in Tfec mutant macrophages. Our study also provides a general definition of the transcriptome in alternatively activated mouse macrophages and identifies a large number of novel genes characterizing this cell type.

Upprunalegt tungumálEnska
Síður (frá-til)7111-7122
Síðufjöldi12
FræðitímaritJournal of Immunology
Bindi174
Númer tölublaðs11
DOI
ÚtgáfustaðaÚtgefið - 1 jún. 2005

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