Toward metabolic phenomics: Analysis of genomic data using flux balances

Christophe H. Schilling, Jeremy S. Edwards, Bernhard O. Palsson

Rannsóknarafurð: Framlag til fræðitímaritsYfirlitsgreinritrýni

154 Tilvitnanir (Scopus)

Útdráttur

Small genome sequencing and annotations are leading to the definition of metabolic genotypes in an increasing number of organisms. Proteomics is beginning to give insights into the use of the metabolic genotype under given growth conditions. These data sets give the basis for systemically studying the genotype-phenotype relationship. Methods of systems science need to be employed to analyze, interpret, and predict this complex relationship. These endeavors will lead to the development of a new field, tentatively named phenomics. This article illustrates how the metabolic characteristics of annotated small genomes can be analyzed using flux balance analysis (FBA). A general algorithm for the formulation of in silico metabolic genotypes is described. Illustrative analyses of the in silico Escherichia coli K-12 metabolic genotypes are used to show how FBA can be used to study the capabilities of this strain.

Upprunalegt tungumálEnska
Síður (frá-til)288-295
Síðufjöldi8
FræðitímaritBiotechnology Progress
Bindi15
Númer tölublaðs3
DOI
ÚtgáfustaðaÚtgefið - maí 1999

Fingerprint

Sökktu þér í rannsóknarefni „Toward metabolic phenomics: Analysis of genomic data using flux balances“. Saman myndar þetta einstakt fingrafar.

Vitna í þetta