The Long Noncoding RNA Landscape of the Ischemic Human Left Ventricle

Louis A. Saddic, Martin I. Sigurdsson, Tzuu Wang Chang, Erica Mazaika, Mahyar Heydarpour, Stanton K. Shernan, Christine E. Seidman, Jon G. Seidman, Sary F. Aranki, Simon C. Body, Jochen D. Muehlschlegel

Rannsóknarafurð: Framlag til fræðitímaritsGreinritrýni

17 Tilvitnanir (Scopus)

Útdráttur

Background - The discovery of functional classes of long noncoding RNAs (lncRNAs) has expanded our understanding of the variety of RNA species that exist in cells. In the heart, lncRNAs have been implicated in the regulation of development, ischemic and dilated cardiomyopathy, and myocardial infarction. Nevertheless, there is a limited description of expression profiles for these transcripts in human subjects. Methods and Results - We obtained left ventricular tissue from human patients undergoing cardiac surgery and used RNA sequencing to describe an lncRNA profile. We then identified a list of lncRNAs that were differentially expressed between pairs of samples before and after the ischemic insult of cardiopulmonary bypass. The expression of some of these lncRNAs correlates with ischemic time. Coding genes in close proximity to differentially expressed lncRNAs and coding genes that have coordinated expression with these lncRNAs are enriched in functional categories related to myocardial infarction, including heart function, metabolism, the stress response, and the immune system. Conclusions - We describe a list of lncRNAs that are differentially expressed after ischemia in the human heart. These genes are predicted to function in pathways consistent with myocardial injury. As a result, lncRNAs may serve as novel diagnostic and therapeutic targets for ischemic heart disease. Clinical Trial Registration - URL: http://www.clinicaltrials.gov. Unique identifier: NCT00985049.

Upprunalegt tungumálEnska
Númer greinar001534
FræðitímaritCirculation: Cardiovascular Genetics
Bindi10
Númer tölublaðs1
DOI
ÚtgáfustaðaÚtgefið - 1 feb. 2017

Athugasemd

Publisher Copyright:
© 2017 American Heart Association, Inc.

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