Scalable method to determine mutations that occur during adaptive evolution of Escherichia coli

Anuradha Raghunathan, Bernhard Palsson

Rannsóknarafurð: Framlag til fræðitímaritsGreinritrýni

7 Tilvitnanir (Scopus)

Útdráttur

Denaturing HPLC was used to determine mutations occurring during the adaptive evolution of Escherichia coli K-12. The strains were evolved over 700 generations on glycerol as the sole carbon source from a sub-optimal to an optimal growth rate. The mutations detected by direct sequencing of amplicons of the glycerol-phosphate regulon repressor (glpR) gene were a synonymous substitution Val20Val in two separately evolved strains. Nonsynonymous substitutions, Val119Gly and Gly179Trp, were also observed in each of the two strains. This procedure can be scaled to determine genome-scale sequence variations that have occurred during adaptive evolution.

Upprunalegt tungumálEnska
Síður (frá-til)435-441
Síðufjöldi7
FræðitímaritBiotechnology Letters
Bindi25
Númer tölublaðs5
DOI
ÚtgáfustaðaÚtgefið - mar. 2003

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