Maternal diet and dioxin-like activity, bulky DNA adducts and micronuclei in mother-newborns

Marie Pedersen, Thorhallur I. Halldorsson, Herman Autrup, Abraham Brouwer, Harrie Besselink, Steffen Loft, Lisbeth E. Knudsen

Rannsóknarafurð: Framlag til fræðitímaritsGreinritrýni

17 Tilvitnanir (Scopus)


Maternal diet can contribute to carcinogenic exposures and also modify effects of environmental exposures on maternal and fetal genetic stability.In this study, associations between maternal diet and the levels of dioxin-like plasma activity, bulky DNA adducts in white blood cells and micronuclei (MN) in lymphocytes from mother to newborns were examined. From 98 pregnant women living in the greater area of Copenhagen, Denmark in 2006-2007, maternal peripheral blood and umbilical cord blood were collected, together with information on health, environmental exposure and lifestyle. Maternal diet was estimated on the basis of maternal food frequency questionnaire (FFQ) completed by the end of pregnancy. Biomarkers were detected in paired blood samples through the dioxin-responsive chemical-activated luciferase expression (CALUX)® bioassay, 32P-postlabelling technique and cytokinesis-block MN assay.Maternal preference for meats with dark surface were significantly associated with higher bulky DNA adducts in both maternal (β 95%CI; 0.46 (0.08, 0.84)) and cord blood (β 95%CI; 0.46 (0.05, 0.86)) before and after adjustment for potential confounders. No other significant associations between the 18 dietary variables and the biomarkers measured in maternal and fetal samples were identified.The present study suggests that maternal intake of meats with dark surface contributes to the bulky DNA adduct levels in maternal and umbilical cord blood. Relationship between food preparation and bulky DNA adducts appear to be captured by a FFQ while potential associations for other biomarkers might be more complex or need larger sample size.

Upprunalegt tungumálEnska
Síður (frá-til)12-19
FræðitímaritMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Númer tölublaðs1-2
ÚtgáfustaðaÚtgefið - 1 jún. 2012


Funding Information:
We thank all participants, the maternal ward personnel at the University Hospital of Copenhagen, Tina Mose, Line Mathiesen from the Department of Public Health, University of Copenhagen and Marie Frederiksen from the National Environmental Research Institute, Aarhus University for assistance in umbilical cord blood collection and Vibeke Munk from the Department of Public Health, University of Copenhagen for proof reading of the manuscript. This work was co-financed by the Faculty of Health, University of Copenhagen and the EU Integrated Project NewGeneris, Sixth Framework Program , Priority 5: Food Quality and Safety (Contract no. FOOD-CT-2005-016320 ). NewGeneris is the acronym of the project ‘Newborns and Genotoxic exposure risks’ ( ).


Sökktu þér í rannsóknarefni „Maternal diet and dioxin-like activity, bulky DNA adducts and micronuclei in mother-newborns“. Saman myndar þetta einstakt fingrafar.

Vitna í þetta