Long patch base excision repair with purified human proteins. DNA ligase I as patch size mediator for DNA polymerases δ and ε

Barbara Pascucci, Manuel Stucki, Zophonías O. Jónsson, Eugenia Dogliotti, Ulrich Hübscher

Rannsóknarafurð: Framlag til fræðitímaritsGreinritrýni

156 Tilvitnanir (Scopus)

Útdráttur

Among the different base excision repair pathways known, the long patch base excision repair of apurinic/apyrimidinic sites is an important mechanism that requires proliferating cell nuclear antigen. We have reconstituted this pathway using purified human proteins. Our data indicated that efficient repair is dependent on six components including AP endonuclease, replication factor C, proliferating cell nuclear antigen, DNA polymerases δ or ε, flap endonuclease 1, and DNA ligase I. Fine mapping of the nucleotide replacement events showed that repair patches extended up to a maximum of 10 nucleotides 3' to the lesion. However, almost 70% of the repair synthesis was confined to 2-4-nucleotide patches and DNA ligase I appeared to be responsible for limiting the repair patch length. Moreover, both proliferating cell nuclear antigen and flap endonuclease 1 are required for the production and ligation of long patch repair intermediates suggesting an important role of this complex in both excision and resynthesis steps.

Upprunalegt tungumálEnska
Síður (frá-til)33696-33702
Síðufjöldi7
FræðitímaritJournal of Biological Chemistry
Bindi274
Númer tölublaðs47
DOI
ÚtgáfustaðaÚtgefið - 19 nóv. 1999

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