Altered expression of autoimmune regulator in infant down syndrome thymus, a possible contributor to an autoimmune phenotype.

Gabriel Skogberg, Vanja Lundberg, Susanne Lindgren, Judith Gudmundsdottir, Kerstin Sandström, Olle Kämpe, Göran Annerén, Jan Gustafsson, Jan Sunnegårdh, Sjoerd van der Post, Esbjörn Telemo, Martin Berglund, Olov Ekwall

Rannsóknarafurð: Framlag til fræðitímaritsGreinritrýni

Útdráttur

Down syndrome (DS), caused by trisomy of chromosome 21, is associated with immunological dysfunctions such as increased frequency of infections and autoimmune diseases. Patients with DS share clinical features, such as autoimmune manifestations and specific autoantibodies, with patients affected by autoimmune polyendocrine syndrome type 1. Autoimmune polyendocrine syndrome type 1 is caused by mutations in the autoimmune regulator (AIRE) gene, located on chromosome 21, which regulates the expression of tissue-restricted Ags (TRAs) in thymic epithelial cells. We investigated the expression of AIRE and TRAs in DS and control thymic tissue using quantitative PCR. AIRE mRNA levels were elevated in thymic tissue from DS patients, and trends toward increased expression of the AIRE-controlled genes INSULIN and CHRNA1 were found. Immunohistochemical stainings showed altered cell composition and architecture of the thymic medulla in DS individuals with increased frequencies of AIRE-positive medullary epithelial cells and CD11c-positive dendritic cells as well as enlarged Hassall's corpuscles. In addition, we evaluated the proteomic profile of thymic exosomes in DS individuals and controls. DS exosomes carried a broader protein pool and also a larger pool of unique TRAs compared with control exosomes. In conclusion, the increased AIRE gene dose in DS could contribute to an autoimmune phenotype through multiple AIRE-mediated effects on homeostasis and function of thymic epithelial cells that affect thymic selection processes.
Upprunalegt tungumálEnska
FræðitímaritJournal of immunology (Baltimore, Md. : 1950)
DOI
ÚtgáfustaðaÚtgefið - 1 sep. 2014

Önnur efnisorð

  • Downs heilkenni
  • Antigens, CD11c
  • Chromosomes, Human, Pair 21
  • Dendritic Cells
  • Down Syndrome
  • Epithelial Cells
  • Exosomes
  • Female
  • Gene Dosage
  • Gene Expression Regulation
  • Humans
  • Immunohistochemistry
  • Infant
  • Infant, Newborn
  • Insulin
  • Male
  • Phenotype
  • RNA, Messenger
  • Receptors, Nicotinic
  • Thymus Gland
  • Transcription Factors

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