Whole-cell patch clamp recordings from oligodendrocyte lineage cells in brain slices

Sylvia Agathou, Ragnhildur Þóra Káradóttir*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Citations (Scopus)


The whole-cell configuration of the patch-clamp technique is widely used to study electrically active cells and passive membrane properties, as well as the properties and pharmacology of ion channels, neurotransmitter receptors, and electrogenic transporters, in almost any cell type. In the brain, in addition to neurons, oligodendrocyte precursor cells (OPCs) that give rise to myelinating oligodendrocytes (OLs) are also excitable. Electrophysiological techniques provide the main tool for the thorough investigation of the electrogenic capacity of such cell types. Although there are many published protocols for whole-cell recordings, there are very few that touch upon the electrophysiological characteristics of oligodendrocyte lineage cells. Here we provide a detailed methodology for how to acquire and analyze whole-cell recordings from excitable cells, with a focus on oligodendrocyte lineage cells. We provide a protocol on how to successfully identify OPCs and OLs in brain slices, either with the use of transgenic animal models or through morphological and electrophysiological profiling. The method described can also be easily adopted for whole-cell recordings from oligodendrocyte lineage cells in vitro

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages28
Publication statusPublished - 2019
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media, LLC, part of Springer Nature 2019.

Other keywords

  • Electrophysiology
  • Myelination
  • Oligodendrocyte precursor cells
  • Oligodendrocytes
  • Whole-cell patch clamp


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