Transcriptome-wide association study of multiple myeloma identifies candidate susceptibility genes

Molly Went, Ben Kinnersley, Amit Sud, David C. Johnson, Niels Weinhold, Asta Försti, Mark van Duin, Giulia Orlando, Jonathan S. Mitchell, Rowan Kuiper, Brian A. Walker, Walter M. Gregory, Per Hoffmann, Graham H. Jackson, Markus M. Nöthen, Miguel Inacio da Silva Filho, Hauke Thomsen, Annemiek Broyl, Faith E. Davies, Unnur ThorsteinsdottirMarkus Hansson, Martin Kaiser, Pieter Sonneveld, Hartmut Goldschmidt, Kari Stefansson, Kari Hemminki, Björn Nilsson, Gareth J. Morgan, Richard S. Houlston

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

BACKGROUND: While genome-wide association studies (GWAS) of multiple myeloma (MM) have identified variants at 23 regions influencing risk, the genes underlying these associations are largely unknown. To identify candidate causal genes at these regions and search for novel risk regions, we performed a multi-tissue transcriptome-wide association study (TWAS). RESULTS: GWAS data on 7319 MM cases and 234,385 controls was integrated with Genotype-Tissue Expression Project (GTEx) data assayed in 48 tissues (sample sizes, N = 80-491), including lymphocyte cell lines and whole blood, to predict gene expression. We identified 108 genes at 13 independent regions associated with MM risk, all of which were in 1 Mb of known MM GWAS risk variants. Of these, 94 genes, located in eight regions, had not previously been considered as a candidate gene for that locus. CONCLUSIONS: Our findings highlight the value of leveraging expression data from multiple tissues to identify candidate genes responsible for GWAS associations which provide insight into MM tumorigenesis. Among the genes identified, a number have plausible roles in MM biology, notably APOBEC3C, APOBEC3H, APOBEC3D, APOBEC3F, APOBEC3G, or have been previously implicated in other malignancies. The genes identified in this TWAS can be explored for follow-up and validation to further understand their role in MM biology.

Original languageEnglish
Pages (from-to)37
Number of pages1
JournalHuman Genomics
Volume13
Issue number1
DOIs
Publication statusPublished - 20 Aug 2019

Bibliographical note

Funding Information:
This work was supported by grants from Myeloma UK, Bloodwise and Cancer Research UK (C1298/A8362). M.W. is supported by funding from Mr. Ralph Stockwell. A.S. is supported by a clinical fellowship from Cancer Research UK and the Royal Marsden Haematology Research Fund.

Other keywords

  • Gene expression
  • Genome-wide association study
  • Multiple myeloma
  • Transcriptome-wide association study

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