TY - JOUR
T1 - Thermus islandicus sp. nov., a mixotrophic sulfur-oxidizing bacterium isolated from the Torfajokull geothermal area
AU - Bjornsdottir, Snaedis H.
AU - Petursdottir, Solveig K.
AU - Hreggvidsson, Gudmundur O.
AU - Skirnisdottir, Sigurlaug
AU - Hjorleifsdottir, Sigridur
AU - Arnfinnsson, Johann
AU - Kristjansson, Jacob K.
PY - 2009
Y1 - 2009
N2 - Strains PRI 2268 and PRI 3838T were isolated from two separate hot springs in the Torfajokull geothermal area of South Iceland. The cells were non-motile rods, approximately 0.3 μm in width and 1.5-2.5 μm in length. Electron microscopy revealed a Gram-negative cell-wall structure. The strains grew at 45-79 °C (optimum, 65 °C) and pH 5.5-10.5 (optimum, pH 6.0-7.0). 16S rRNA gene sequence analysis indicated that they formed a separate branch within the genus Thermus with 'Thermus kawarayensis' KW11 as their closest cultured relative (96.5 % similarity). The gene sequence similarities of both new isolates to Thermus aquaticus YT-1T and Thermus igniterrae RF-4T were 96.1 % and 95.5 %, respectively. DNA-DNA relatedness between strain PRI 3838T and 'T. kawarayensis' was 46.1 %. The DNA G+C content of strain PRI 3838T was 69.0 mol%. The predominant menaquinones, pigmentation, fatty acid profiles and phospholipid profiles of the novel strains were similar to those of other members of the genus Thermus. However, the new strains could be differentiated from the type strains of all other species of the genus Thermus by their lack of catalase activity and their utilization of only a few carbon sources. Furthermore, the novel strains exhibited mixotrophic growth with sulfur oxidation. On the basis of 16S rRNA gene sequence comparisons, DNA-DNA hybridization and physiological and biochemical characteristics, the new isolates represent a novel species. Since the species appears to be ubiquitous in Icelandic hot springs, the name Thermus islandicus sp. nov. is proposed. The type strain is PRI 3838T (=DSM 21543T=ATCC BAA-1677T).
AB - Strains PRI 2268 and PRI 3838T were isolated from two separate hot springs in the Torfajokull geothermal area of South Iceland. The cells were non-motile rods, approximately 0.3 μm in width and 1.5-2.5 μm in length. Electron microscopy revealed a Gram-negative cell-wall structure. The strains grew at 45-79 °C (optimum, 65 °C) and pH 5.5-10.5 (optimum, pH 6.0-7.0). 16S rRNA gene sequence analysis indicated that they formed a separate branch within the genus Thermus with 'Thermus kawarayensis' KW11 as their closest cultured relative (96.5 % similarity). The gene sequence similarities of both new isolates to Thermus aquaticus YT-1T and Thermus igniterrae RF-4T were 96.1 % and 95.5 %, respectively. DNA-DNA relatedness between strain PRI 3838T and 'T. kawarayensis' was 46.1 %. The DNA G+C content of strain PRI 3838T was 69.0 mol%. The predominant menaquinones, pigmentation, fatty acid profiles and phospholipid profiles of the novel strains were similar to those of other members of the genus Thermus. However, the new strains could be differentiated from the type strains of all other species of the genus Thermus by their lack of catalase activity and their utilization of only a few carbon sources. Furthermore, the novel strains exhibited mixotrophic growth with sulfur oxidation. On the basis of 16S rRNA gene sequence comparisons, DNA-DNA hybridization and physiological and biochemical characteristics, the new isolates represent a novel species. Since the species appears to be ubiquitous in Icelandic hot springs, the name Thermus islandicus sp. nov. is proposed. The type strain is PRI 3838T (=DSM 21543T=ATCC BAA-1677T).
UR - http://www.scopus.com/inward/record.url?scp=72149084928&partnerID=8YFLogxK
U2 - 10.1099/ijs.0.007013-0
DO - 10.1099/ijs.0.007013-0
M3 - Article
C2 - 19628590
AN - SCOPUS:72149084928
SN - 1466-5026
VL - 59
SP - 2962
EP - 2966
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 12
ER -