The "fish & chips" project: Microarrays as a tool for the identification of marine organisms in biodiversity and ecosystem research

Marc Kochzius*, Kristina Kappel, Lutz Döbitz, Nina Silkenbeumer, Manfred Nölte, Hannes Weber, Sigridur Hjörleifsdottir, Viggo Marteinsson, Gudmundur Hreggvidsson, Serge Planes, Fausto Tinti, Antonios Magoulas, Eva Garcia Vazquez, Cemal Turan, Linda Medlin, Katja Metfies, Christine Gescher, Alessia Cariani, Monica Landi, Caroline HervetDaniel Campo Falgueras, Aglaia Antoniou, Fabio Bertasi, Chitipothu Srujana, Dietmar Blohm, Guðmundur Óli Hreggviðsson

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

3 Citations (Scopus)

Abstract

DNA microarrays are currently in use almost exclusively as research tools for gene expression analysis and their application for the identification of organisms is still in its infancy, only documented by a few studies on mammals, bacteria, and viruses. The "Fish & Chips" project aims to demonstrate that DNA chips can be a new innovative tool for the identification of marine animals and phytoplankton. To achieve this goal, fishes, invertebrates, and phytoplankton were sampled in European seas and taxonomically classified. Fragments of their mitochondrial 16S, cyt b, and COI genes and from the nuclear 18S genes were sequenced and served as molecular markers to enable probe design for the microarrays. An on-line data base containing the sequences and all relevant information of the samples has been implemented. A first prototype of a "Fish Chip",is based on more than 400 sequences from the 16S rRNA gene belonging to 46 species. A second microarray prototype serves to identify flatfishes from the North Sea based on COI-and 16S sequences from 70 individuals of 17 fish species. A "Phytoplankton Chip" is now available with probes for all microalgal classes and many toxic species, as well as a "Invertebrate Chip". The results show that this approach is feasible.

Original languageEnglish
Title of host publicationOCEANS 2007 - Europe
Publication statusPublished - 2007
EventOCEANS 2007 - Europe - Aberdeen, Scotland, United Kingdom
Duration: 18 Jun 200721 Jun 2007

Publication series

NameOCEANS 2007 - Europe

Conference

ConferenceOCEANS 2007 - Europe
Country/TerritoryUnited Kingdom
CityAberdeen, Scotland
Period18/06/0721/06/07

Other keywords

  • DNA barcoding
  • DNA chips
  • European seas
  • Genetic identification

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