Abstract
Heating and changes in pH often practised during fish protein hydrolysis can cause lipid oxidation. The effect of natural antioxidants towards haemoglobin-mediated lipid oxidation during enzymatic hydrolysis of cod proteins was investigated. Different variants of a washed cod model system, containing different combinations of haemoglobin and natural antioxidants (L-ascorbic acid and Fuscus vesiculosus extract), were hydrolysed using Protease P "Amano" 6 at pH 8 and 36°C to achieve 20% degree of hydrolysis. Lipid hydroperoxides and thiobarbituric acid reactive substances (TBARS) were analysed periodically during the hydrolysis process. The in vitro antioxidant activity of the final products was investigated. Results indicate that oxidation can develop rapidly during hydrolysis and antioxidant strategies are preferable to produce good quality products. Oxidation products did not have an impact on the in vitro antioxidant activity of the hydrolysates. The natural antioxidants inhibited oxidation during hydrolysis and contributed to the antioxidant activity of the final product.
| Original language | English |
|---|---|
| Pages (from-to) | 914-919 |
| Number of pages | 6 |
| Journal | Food Chemistry |
| Volume | 141 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2013 |
Bibliographical note
Funding Information:This project was funded by the AVS research fund under the Ministry of Fisheries in Iceland (project no. R-047-07). The financing of this work is gratefully acknowledged. The authors would also like to thank the associate professor Kazufumi Osako from Tokyo University of Marine Science and Technology for his support and Amano Enzyme Inc. for kindly providing Protease P Amano 6.
Other keywords
- Enzyme hydrolysis
- Fish protein hydrolysates
- Lipid oxidation
- Natural antioxidants