The barcode, UMI, set format and BUStools

Páll Melsted*, Vasilis Ntranos, Lior Pachter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Summary: We introduce the Barcode-UMI-Set format (BUS) for representing pseudoalignments of reads from single-cell RNA-seq experiments. The format can be used with all single-cell RNA-seq technologies, and we show that BUS files can be efficiently generated. BUStools is a suite of tools for working with BUS files and facilitates rapid quantification and analysis of single-cell RNA-seq data. The BUS format therefore makes possible the development of modular, technology-specific and robust workflows for single-cell RNA-seq analysis. Availability and implementation: and Supplementary information: Supplementary data are available at Bioinformatics online.

Original languageEnglish
Pages (from-to)4472-4473
Number of pages2
Issue number21
Publication statusPublished - 1 Nov 2019

Bibliographical note

Publisher Copyright:
© 2019 The Author(s) 2019. Published by Oxford University Press. All rights reserved.


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