Temperature significantly affects retroviral vector production and deactivation rates, and thereby determines retroviral titers

G. M. Lee*, J. H. Choi, S. C. Jun, B. O. Palsson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

The retroviral titer obtained from the pMFG/ψCRIP producer cell line is determined by a dynamic interplay of vector production and deactivation rates. Both these rates are influenced by temperature. It was determined that; (i) the retroviral half-lives are strongly influenced by temperature and the temperature dependency can be described by the Arrhenius equation with an activation energy of 39 kcal/gmol; (ii) the actual retroviral vector productivity per cell is highest at 37°C with retroviral production rate of 24.4(±7.0; ± standard deviation) colony forming unit (CFU)/cell/day; (iii) the dynamic interplay of these two factors produces an optimal temperature of 34°C for pMFG/ψCRIP cells under the culture conditions used; and (iv) the cellular growth rate is highest at 37°C at 26.8 hr doubling time. Taken together, these parameters can be used to optimize a two-step retroviral production protocol, where the cells are first grown under optimal growth conditions (37°C) and second, the virus is produced at 34°C to yield the highest titer. These results have significant implications for optimal retroviral production protocols.

Original languageEnglish
Pages (from-to)343-349
Number of pages7
JournalBioprocess Engineering
Volume19
Issue number5
DOIs
Publication statusPublished - 1998

Bibliographical note

Funding Information:
The authors would like to thank Dr. J.M. Wilson for providing the packaging cell line. This work was supported in part by Korea Science and Engineering Foundation (Grant No. 951-1104-010-1).

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