Synthesis and characterization of RNA containing a rigid and nonperturbing cytidine-derived spin label

Claudia Höbartner; Giuseppe Sicoli; Falk Wachowius; Dnyaneshwar B. Gophane; Snorri Th Sigurdsson

doi:10.1021/jo301227w

Synthesis and characterization of RNA containing a rigid and nonperturbing cytidine-derived spin label

Claudia Höbartner^*, Giuseppe Sicoli, Falk Wachowius, Dnyaneshwar B. Gophane, Snorri Th Sigurdsson

^*Corresponding author for this work

Faculty of Physical Sciences

Research output: Contribution to journal › Article › peer-review

47 Citations (Scopus)

Abstract

The nitroxide-containing nucleoside Çm is reported as the first rigid spin label for paramagnetic modification of RNA by solid-phase synthesis. The spin label is well accommodated in several RNA secondary structures as judged by its minor effect on the thermodynamic stability of hairpin and duplex RNA. Electron paramagnetic resonance (EPR) spectroscopic characterization of mono-, bi-, and trimolecular RNA structures shows that Çm will be applicable for advanced EPR studies to elucidate structural and dynamic aspects of folded RNA.

Original language	English
Pages (from-to)	7749-7754
Number of pages	6
Journal	Journal of Organic Chemistry
Volume	77
Issue number	17
DOIs	https://doi.org/10.1021/jo301227w
Publication status	Published - 7 Sept 2012

Access to Document

10.1021/jo301227w

Cite this

@article{eeccacca35b14cab9a91a2a5b3307123,

title = "Synthesis and characterization of RNA containing a rigid and nonperturbing cytidine-derived spin label",

abstract = "The nitroxide-containing nucleoside {\c C}m is reported as the first rigid spin label for paramagnetic modification of RNA by solid-phase synthesis. The spin label is well accommodated in several RNA secondary structures as judged by its minor effect on the thermodynamic stability of hairpin and duplex RNA. Electron paramagnetic resonance (EPR) spectroscopic characterization of mono-, bi-, and trimolecular RNA structures shows that {\c C}m will be applicable for advanced EPR studies to elucidate structural and dynamic aspects of folded RNA.",

author = "Claudia H{\"o}bartner and Giuseppe Sicoli and Falk Wachowius and Gophane, {Dnyaneshwar B.} and Sigurdsson, {Snorri Th}",

year = "2012",

month = sep,

day = "7",

doi = "10.1021/jo301227w",

language = "English",

volume = "77",

pages = "7749--7754",

journal = "Journal of Organic Chemistry",

issn = "0022-3263",

publisher = "American Chemical Society",

number = "17",

}

TY - JOUR

T1 - Synthesis and characterization of RNA containing a rigid and nonperturbing cytidine-derived spin label

AU - Höbartner, Claudia

AU - Sicoli, Giuseppe

AU - Wachowius, Falk

AU - Gophane, Dnyaneshwar B.

AU - Sigurdsson, Snorri Th

PY - 2012/9/7

Y1 - 2012/9/7

N2 - The nitroxide-containing nucleoside Çm is reported as the first rigid spin label for paramagnetic modification of RNA by solid-phase synthesis. The spin label is well accommodated in several RNA secondary structures as judged by its minor effect on the thermodynamic stability of hairpin and duplex RNA. Electron paramagnetic resonance (EPR) spectroscopic characterization of mono-, bi-, and trimolecular RNA structures shows that Çm will be applicable for advanced EPR studies to elucidate structural and dynamic aspects of folded RNA.

AB - The nitroxide-containing nucleoside Çm is reported as the first rigid spin label for paramagnetic modification of RNA by solid-phase synthesis. The spin label is well accommodated in several RNA secondary structures as judged by its minor effect on the thermodynamic stability of hairpin and duplex RNA. Electron paramagnetic resonance (EPR) spectroscopic characterization of mono-, bi-, and trimolecular RNA structures shows that Çm will be applicable for advanced EPR studies to elucidate structural and dynamic aspects of folded RNA.

UR - http://www.scopus.com/inward/record.url?scp=84866058511&partnerID=8YFLogxK

U2 - 10.1021/jo301227w

DO - 10.1021/jo301227w

M3 - Article

C2 - 22881715

AN - SCOPUS:84866058511

SN - 0022-3263

VL - 77

SP - 7749

EP - 7754

JO - Journal of Organic Chemistry

JF - Journal of Organic Chemistry

IS - 17

ER -

Synthesis and characterization of RNA containing a rigid and nonperturbing cytidine-derived spin label

Abstract

Access to Document

Fingerprint

Cite this