Streptomyces as Microbial Chassis for Heterologous Protein Expression

Soonkyu Hwang, Yongjae Lee, Ji Hun Kim, Gahyeon Kim, Hyeseong Kim, Woori Kim, Suhyung Cho, Bernhard O. Palsson, Byung Kwan Cho*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review


Heterologous production of recombinant proteins is gaining increasing interest in biotechnology with respect to productivity, scalability, and wide applicability. The members of genus Streptomyces have been proposed as remarkable hosts for heterologous production due to their versatile nature of expressing various secondary metabolite biosynthetic gene clusters and secretory enzymes. However, there are several issues that limit their use, including low yield, difficulty in genetic manipulation, and their complex cellular features. In this review, we summarize rational engineering approaches to optimizing the heterologous production of secondary metabolites and recombinant proteins in Streptomyces species in terms of genetic tool development and chassis construction. Further perspectives on the development of optimal Streptomyces chassis by the design-build-test-learn cycle in systems are suggested, which may increase the availability of secondary metabolites and recombinant proteins.

Original languageEnglish
Article number804295
JournalFrontiers in Bioengineering and Biotechnology
Publication statusPublished - 21 Dec 2021

Bibliographical note

Publisher Copyright:
Copyright © 2021 Hwang, Lee, Kim, Kim, Kim, Kim, Cho, Palsson and Cho.

Other keywords

  • chassis
  • heterologous expression
  • recombinant protein
  • secondary metabolite
  • streptomyces


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