Retrotransposon BARE-1 and its role in genome evolution in the genus Hordeum

Carlos M. Vicient, Annu Suoniemi, Kesara Anamthawat-Jonsson, Jaakko Tanskanen, Alex Beharav, Eviatar Nevo, Alan H. Schulman*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

295 Citations (Scopus)

Abstract

The replicative retrotransposon life cycle offers the potential for explosive increases in copy number and consequent inflation of genome size. The BARE-1 retrotransposon family of barley is conserved, disperse, and transcriptionally active. To assess the role of BARE-1 in genome evolution, we determined the copy number of its integrase, its reverse transcriptase, and its long terminal repeat (LTR) domains throughout the genus Hordeum. On average, BARE-1 contributes 13.7 x 103 full-length copies, amounting to 2.9% of the genome. The number increases with genome size. Two LTRs are associated with each internal domain in intact retrotransposons, but surprisingly, BARE-1 LTRs were considerably more prevalent than would be expected from the numbers of intact elements. The excess in LTRs increases as both genome size and BARE-1 genomic fraction decrease. Intrachromosomal homologous recombination between LTRs could explain the excess, removing BARE-1 elements and leaving behind solo LTRs, thereby reducing the complement of functional retrotransposons in the genome and providing at least a partial 'return ticket from genomic obesity'.

Original languageEnglish
Pages (from-to)1769-1784
Number of pages16
JournalPlant Cell
Volume11
Issue number9
DOIs
Publication statusPublished - Sept 1999

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