Recombinant trypsin Y from atlantic cod-properties for commercial use

Helga Margrét Pálsdóttir, Ágústa Gudmundsdóttir*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


This paper describes the production of the novel recombinant trypsin Y polypeptide from Atlantic cod (Gadus morhua) in a Pichia pastoris expression system and gives a characterization of the enzyme. It presents the first biochemical data supplementing the molecular description of the putative group III trypsins. Unexpectedly, the results demonstrate that the recombinant trypsin Y has a dual substrate specificity demonstrating both trypsin and chymotrypsin activities. It shows increasing proteolytic activity at temperatures between 2-21°C, and it is completely inactivated at 30°C. These properties may be important for the commercial use of recombinant trypsin Y, as other cold-adapted proteolytic enzymes from the Atlantic cod have proven their usefulness in various industrial and medical applications.

Original languageEnglish
Pages (from-to)85-100
Number of pages16
JournalJournal of Aquatic Food Product Technology
Issue number2
Publication statusPublished - 2004

Other keywords

  • Atlantic cod
  • Cold-adapted
  • Expression
  • Group III trypsin
  • Serine protease


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