Re-classification of Streptomyces venezuelae strains and mining secondary metabolite biosynthetic gene clusters

Namil Lee, Mira Choi, Woori Kim, Soonkyu Hwang, Yongjae Lee, Ji Hun Kim, Gahyeon Kim, Hyeseong Kim, Suhyung Cho, Sun Chang Kim, Bernhard Palsson, Kyoung Soon Jang*, Byung Kwan Cho

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Streptomyces species have attracted considerable interest as a reservoir of medically important secondary metabolites, which are even diverse and different between strains. Here, we reassess ten Streptomyces venezuelae strains by presenting the highly resolved classification, using 16S rRNA sequencing, MALDI-TOF MS protein profiling, and whole-genome sequencing. The results revealed that seven of the ten strains were misclassified as S. venezuelae species. Secondary metabolite biosynthetic gene cluster (smBGC) mining and targeted LC-MS/MS based metabolite screening of S. venezuelae and misclassified strains identified in total 59 secondary metabolites production. In addition, a comparison of pyrrolamide-type antibiotic BGCs of four misclassified strains, followed by functional genomics, revealed that athv28 is critical in the synthesis of the anthelvencin precursor, 5-amino-3,4-dihydro-2H-pyrrole-2-carboxylate (ADPC). Our findings illustrate the importance of the accurate classification and better utilization of misclassified Streptomyces strains to discover smBGCs and their secondary metabolite products.

Original languageEnglish
Article number103410
Pages (from-to)103410
Issue number12
Publication statusPublished - 1 Nov 2021

Bibliographical note

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Other keywords

  • Metabolomics
  • Microbial genomics
  • Microbiology
  • Phylogeny


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