Prdm14 promotes germline fate and naive pluripotency by repressing FGF signalling and DNA methylation

Nils Grabole; Julia Tischler; Jamie A. Hackett; Shinseog Kim; Fuchou Tang; Harry G. Leitch; Erna Magnúsdóttir; M. Azim Surani

doi:10.1038/embor.2013.67

Prdm14 promotes germline fate and naive pluripotency by repressing FGF signalling and DNA methylation

Nils Grabole, Julia Tischler, Jamie A. Hackett, Shinseog Kim, Fuchou Tang, Harry G. Leitch, Erna Magnúsdóttir, M. Azim Surani^*

^*Corresponding author for this work

Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

112 Citations (Scopus)

Abstract

Primordial germ cells (PGCs) and somatic cells originate from postimplantation epiblast cells in mice. As pluripotency is lost upon differentiation of somatic lineages, a naive epigenome and the pluripotency network are re-established during PGC development. Here we demonstrate that Prdm14 contributes not only to PGC specification, but also to naive pluripotency in embryonic stem (ES) cells by repressing the DNA methylation machinery and fibroblast growth factor (FGF) signalling. This indicates a critical role for Prdm14 in programming PGCs and promoting pluripotency in ES cells.

Original language	English
Pages (from-to)	629-637
Number of pages	9
Journal	EMBO Reports
Volume	14
Issue number	7
DOIs	https://doi.org/10.1038/embor.2013.67
Publication status	Published - Jun 2013

Other keywords

DNA methylation
FGF signalling
pluripotency
Prdm14
primordial germ cells

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10.1038/embor.2013.67

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abstract = "Primordial germ cells (PGCs) and somatic cells originate from postimplantation epiblast cells in mice. As pluripotency is lost upon differentiation of somatic lineages, a naive epigenome and the pluripotency network are re-established during PGC development. Here we demonstrate that Prdm14 contributes not only to PGC specification, but also to naive pluripotency in embryonic stem (ES) cells by repressing the DNA methylation machinery and fibroblast growth factor (FGF) signalling. This indicates a critical role for Prdm14 in programming PGCs and promoting pluripotency in ES cells.",

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AU - Grabole, Nils

AU - Tischler, Julia

AU - Hackett, Jamie A.

AU - Kim, Shinseog

AU - Tang, Fuchou

AU - Leitch, Harry G.

AU - Magnúsdóttir, Erna

AU - Surani, M. Azim

PY - 2013/6

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AB - Primordial germ cells (PGCs) and somatic cells originate from postimplantation epiblast cells in mice. As pluripotency is lost upon differentiation of somatic lineages, a naive epigenome and the pluripotency network are re-established during PGC development. Here we demonstrate that Prdm14 contributes not only to PGC specification, but also to naive pluripotency in embryonic stem (ES) cells by repressing the DNA methylation machinery and fibroblast growth factor (FGF) signalling. This indicates a critical role for Prdm14 in programming PGCs and promoting pluripotency in ES cells.

KW - DNA methylation

KW - FGF signalling

KW - pluripotency

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Prdm14 promotes germline fate and naive pluripotency by repressing FGF signalling and DNA methylation

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