Population studies and validation of paternity determinations by six microsatellite loci

Sigurdur Ingvarsson*, Valdis Finnsdottir, Asgeir Sigurdsson, Gunnlaugur Geirsson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)


A single locus system of 6 microsatellite markers was evaluated for paternity testing. A nonradioactive method based on peroxidase labeling of a DNA probe was used to estimate the allele frequency of markers D1S216, D3S1217, D7S480, D9S157, D13S153, and D16S422 by genotyping 1134-1698 chromosomes. The number of detected alleles were 22, 15, 23, 10, 16, and 19, respectively, and the allele frequency varied from 0.001 to 0.317. The genotype of 87 families, consisting of mother, father, and child was determined. The probability that a random individual will give a positive paternity was evaluated. We conclude that the markers can be reliably typed and give sufficient and reliable information for paternity testing.

Original languageEnglish
Pages (from-to)692-695
Number of pages4
JournalJournal of Forensic Sciences
Issue number3
Publication statusPublished - May 2000

Other keywords

  • D13S153
  • D16S422
  • D1S216
  • D3S1217
  • D7S480
  • D9S157
  • Dinucleotide
  • Forensic DNA typing
  • Forensic science
  • Paternity
  • Polymerase chain reaction
  • Short tandem repeat


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