TY - JOUR
T1 - Polyphasic analysis of Thermus isolates from geothermal areas in Iceland
AU - Hreggvidsson, Gudmundur O.
AU - Skirnisdottir, Sigurlaug
AU - Smit, Bart
AU - Hjorleifsdottir, Sigridur
AU - Marteinsson, Viggo Th
AU - Petursdottir, Solveig
AU - Kristjansson, Jakob K.
PY - 2006/12
Y1 - 2006/12
N2 - Genetic relationships and diversity of 101 Thermus isolates from different geothermal regions in Iceland were investigated by using multilocus enzyme electrophoresis (MLEE) and small subunit ribosomal rRNA (SSU rRNA) sequence analysis. Ten polymorphic enzymes were used and seven distinct and genetically highly divergent lineages of Thermus were observed. Six of seven lineages could be assigned to species whose names have been validated. The most diverse lineage was Thermus scotoductus. In contrast to the other lineages, this lineage was divided into very distinct genetic sublineages that may represent subspecies with different habitat preferences. The least diverse lineage was Thermus brockianus. Phenotypic and physiological analysis was carried out on a subset of the isolates. No relationship was found between growth on specific single carbon source to the grouping obtained by the isoenzyme analysis. The response to various salts was distinguishing in a few cases. No relationship was found between temperature at the isolation site and the different lineages, but pH indicated a relation to specific lineages.
AB - Genetic relationships and diversity of 101 Thermus isolates from different geothermal regions in Iceland were investigated by using multilocus enzyme electrophoresis (MLEE) and small subunit ribosomal rRNA (SSU rRNA) sequence analysis. Ten polymorphic enzymes were used and seven distinct and genetically highly divergent lineages of Thermus were observed. Six of seven lineages could be assigned to species whose names have been validated. The most diverse lineage was Thermus scotoductus. In contrast to the other lineages, this lineage was divided into very distinct genetic sublineages that may represent subspecies with different habitat preferences. The least diverse lineage was Thermus brockianus. Phenotypic and physiological analysis was carried out on a subset of the isolates. No relationship was found between growth on specific single carbon source to the grouping obtained by the isoenzyme analysis. The response to various salts was distinguishing in a few cases. No relationship was found between temperature at the isolation site and the different lineages, but pH indicated a relation to specific lineages.
KW - Hot springs
KW - Iceland
KW - Multilocus enzyme electrophoresis
KW - Polyphasic taxonomic
KW - Thermus
UR - http://www.scopus.com/inward/record.url?scp=33751098229&partnerID=8YFLogxK
U2 - 10.1007/s00792-006-0530-3
DO - 10.1007/s00792-006-0530-3
M3 - Article
C2 - 16799746
AN - SCOPUS:33751098229
VL - 10
SP - 563
EP - 575
JO - Extremophiles
JF - Extremophiles
SN - 1431-0651
IS - 6
ER -