Orientation selection in distance measurements between nitroxide spin labels at 94 GHz EPR with variable dual frequency irradiation

Igor Tkach, Soraya Pornsuwan, Claudia Höbartner, Falk Wachowius, Snorri Th Sigurdsson, Tatiana Y. Baranova, Ulf Diederichsen, Giuseppe Sicoli, Marina Bennati*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

46 Citations (Scopus)

Abstract

Pulsed electron-electron double resonance (PELDOR, also known as DEER) has become a method of choice to measure distances in biomolecules. In this work we show how the performance of the method can be improved at high EPR frequencies (94 GHz) using variable dual frequency irradiation in a dual mode cavity in order to obtain enhanced resolution toward orientation selection. Dipolar evolution traces of a representative RNA duplex and an α-helical peptide were analysed in terms of possible bi-radical structures by considering the inherent ambiguity of symmetry-related solutions. This journal is

Original languageEnglish
Pages (from-to)3433-3437
Number of pages5
JournalPhysical Chemistry Chemical Physics
Volume15
Issue number10
DOIs
Publication statusPublished - 14 Mar 2013

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