TY - JOUR
T1 - Nine new polymorphic microsatellite loci for the amplification of archived otolith DNA of Atlantic cod, Gadus morhua L.
AU - Jakobsdóttir, Klara Björg
AU - Jörundsdóttir, Þóra Dögg
AU - Skírnisdóttir, Sigurlaug
AU - Hjörleifsdóttir, Sigrídur
AU - Hreggvidsson, Gudmundur Ó
AU - Daníelsdóttir, Anna Kristín
AU - Pampoulie, Christophe
PY - 2006/6
Y1 - 2006/6
N2 - Nine out of 22 microsatellite primers tested were successfully amplified on three samples of cod Gadus morhua L. (two contemporary and one archived otolith samples). All loci were polymorphic (5-23 alleles/locus). The average observed heterozygosity across loci and samples was 0.625, ranging from 0.294 to 0.895 at each locus. All loci were under Hardy-Weinberg equilibrium, except PGmo56 that showed significant excess of heterozygotes in all studied samples. The isolated loci were suitable for degraded DNA and therefore useful for conducting a long-term temporal study with DNA obtained from archived otoliths of cod.
AB - Nine out of 22 microsatellite primers tested were successfully amplified on three samples of cod Gadus morhua L. (two contemporary and one archived otolith samples). All loci were polymorphic (5-23 alleles/locus). The average observed heterozygosity across loci and samples was 0.625, ranging from 0.294 to 0.895 at each locus. All loci were under Hardy-Weinberg equilibrium, except PGmo56 that showed significant excess of heterozygotes in all studied samples. The isolated loci were suitable for degraded DNA and therefore useful for conducting a long-term temporal study with DNA obtained from archived otoliths of cod.
KW - Archived DNA
KW - Atlantic cod
KW - Gadus morhua
KW - Microsatellite
UR - http://www.scopus.com/inward/record.url?scp=33646882184&partnerID=8YFLogxK
U2 - 10.1111/j.1471-8286.2005.01223.x
DO - 10.1111/j.1471-8286.2005.01223.x
M3 - Article
AN - SCOPUS:33646882184
SN - 1471-8278
VL - 6
SP - 337
EP - 339
JO - Molecular Ecology Notes
JF - Molecular Ecology Notes
IS - 2
ER -