Mucosal immunization against ovine lentivirus using PEI-DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes

R. Reina; C. Barbezange; H. Niesalla; X. de Andrés; Hallgrímur Arnarson; E. Biescas; M. Mazzei; C. Fraisier; T. N. McNeilly; C. Liu; M. Perez; M. L. Carrozza; P. Bandecchi; C. Solano; H. Crespo; I. Glaria; C. Huard; D. J. Shaw; I. de Blas; D. de Andrés; F. Tolari; S. Rosati; M. Suzan-Monti; Valgerður Andrésdóttir; Sigurbjörg Þorsteinsdóttir; Gudmundur Pétursson; L. Lujan; M. Pepin; B. Amorena; B. Blacklaws; G. D. Harkiss

doi:10.1016/j.vaccine.2008.06.065

Mucosal immunization against ovine lentivirus using PEI-DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes

R. Reina, C. Barbezange, H. Niesalla, X. de Andrés, Hallgrímur Arnarson, E. Biescas, M. Mazzei, C. Fraisier, T. N. McNeilly, C. Liu, M. Perez, M. L. Carrozza, P. Bandecchi, C. Solano, H. Crespo, I. Glaria, C. Huard, D. J. Shaw, I. de Blas, D. de AndrésF. Tolari, S. Rosati, M. Suzan-Monti, Valgerður Andrésdóttir, Sigurbjörg Þorsteinsdóttir, Gudmundur Pétursson, L. Lujan, M. Pepin, B. Amorena, B. Blacklaws, G. D. Harkiss^*

^*Corresponding author for this work

The Institute for Experimental Pathology University of Iceland

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Sheep were immunized against Visna/Maedi virus (VMV) gag and/or env genes via the nasopharynx-associated lymphoid tissue (NALT) and lung using polyethylenimine (PEI)-DNA complexes and modified vaccinia Ankara, and challenged with live virus via the lung. env immunization enhanced humoral responses prior to but not after VMV challenge. Systemic T cell proliferative and cytotoxic responses were generally low, with the responses following single gag gene immunization being significantly depressed after challenge. A transient reduction in provirus load in the blood early after challenge was observed following env immunization, whilst the gag gene either alone or in combination with env resulted in significantly elevated provirus loads in lung. However, despite this, a significant reduction in lesion score was observed in animals immunized with the single gag gene at post-mortem. Inclusion of IFN-γ in the immunization mixture in general had no significant effects. The results thus showed that protective effects against VMV-induced lesions can be induced following respiratory immunization with the single gag gene, though this was accompanied by an increased pulmonary provirus load.

Original language	English
Pages (from-to)	4494-4505
Number of pages	12
Journal	Vaccine
Volume	26
Issue number	35
DOIs	https://doi.org/10.1016/j.vaccine.2008.06.065
Publication status	Published - 18 Aug 2008

Bibliographical note

Funding Information:
This study was supported by grants from European Union (QLK2-CT-2002-00617) and Spanish CICYT (AGL2003-08977-C03-01 and AGL2006-13410C-06/01/GAN). Ramsés Reina and Ximena de Andrés were supported by a fellowship FPI from the Spanish Ministry of Science and Education. Tom McNeilly was supported by a Ph.D. studentship from the Royal (Dick) College of Veterinary Studies, University of Edinburgh. We thank Margaret Ross, Pauline Love, Paolo Caldato, Paul Tonks, Santiago Becerra, Rosario Puyó, Margherita Profiti, Katia Ricci, Giorgia Tozzini, Angela Wheatley, Sigridur Matthiasdottir, and Paul Wright for technical help.

Other keywords

DNA vaccination
Lentivirus
Mucosal
Sheep

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10.1016/j.vaccine.2008.06.065

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Reina, R., Barbezange, C., Niesalla, H., de Andrés, X., Arnarson, H., Biescas, E., Mazzei, M., Fraisier, C., McNeilly, T. N., Liu, C., Perez, M., Carrozza, M. L., Bandecchi, P., Solano, C., Crespo, H., Glaria, I., Huard, C., Shaw, D. J., de Blas, I., ... Harkiss, G. D. (2008). Mucosal immunization against ovine lentivirus using PEI-DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes. Vaccine, 26(35), 4494-4505. https://doi.org/10.1016/j.vaccine.2008.06.065

@article{48881f2c280c4c1e9c8860f418301f61,

title = "Mucosal immunization against ovine lentivirus using PEI-DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes",

abstract = "Sheep were immunized against Visna/Maedi virus (VMV) gag and/or env genes via the nasopharynx-associated lymphoid tissue (NALT) and lung using polyethylenimine (PEI)-DNA complexes and modified vaccinia Ankara, and challenged with live virus via the lung. env immunization enhanced humoral responses prior to but not after VMV challenge. Systemic T cell proliferative and cytotoxic responses were generally low, with the responses following single gag gene immunization being significantly depressed after challenge. A transient reduction in provirus load in the blood early after challenge was observed following env immunization, whilst the gag gene either alone or in combination with env resulted in significantly elevated provirus loads in lung. However, despite this, a significant reduction in lesion score was observed in animals immunized with the single gag gene at post-mortem. Inclusion of IFN-γ in the immunization mixture in general had no significant effects. The results thus showed that protective effects against VMV-induced lesions can be induced following respiratory immunization with the single gag gene, though this was accompanied by an increased pulmonary provirus load.",

keywords = "DNA vaccination, Lentivirus, Mucosal, Sheep",

author = "R. Reina and C. Barbezange and H. Niesalla and {de Andr{\'e}s}, X. and Hallgr{\'i}mur Arnarson and E. Biescas and M. Mazzei and C. Fraisier and McNeilly, {T. N.} and C. Liu and M. Perez and Carrozza, {M. L.} and P. Bandecchi and C. Solano and H. Crespo and I. Glaria and C. Huard and Shaw, {D. J.} and {de Blas}, I. and {de Andr{\'e}s}, D. and F. Tolari and S. Rosati and M. Suzan-Monti and Valger{\dh}ur Andr{\'e}sd{\'o}ttir and Sigurbj{\"o}rg {\TH}orsteinsd{\'o}ttir and Gudmundur P{\'e}tursson and L. Lujan and M. Pepin and B. Amorena and B. Blacklaws and Harkiss, {G. D.}",

note = "Funding Information: This study was supported by grants from European Union (QLK2-CT-2002-00617) and Spanish CICYT (AGL2003-08977-C03-01 and AGL2006-13410C-06/01/GAN). Rams{\'e}s Reina and Ximena de Andr{\'e}s were supported by a fellowship FPI from the Spanish Ministry of Science and Education. Tom McNeilly was supported by a Ph.D. studentship from the Royal (Dick) College of Veterinary Studies, University of Edinburgh. We thank Margaret Ross, Pauline Love, Paolo Caldato, Paul Tonks, Santiago Becerra, Rosario Puy{\'o}, Margherita Profiti, Katia Ricci, Giorgia Tozzini, Angela Wheatley, Sigridur Matthiasdottir, and Paul Wright for technical help.",

year = "2008",

month = aug,

day = "18",

doi = "10.1016/j.vaccine.2008.06.065",

language = "English",

volume = "26",

pages = "4494--4505",

journal = "Vaccine",

issn = "0264-410X",

publisher = "Elsevier BV",

number = "35",

}

Reina, R, Barbezange, C, Niesalla, H, de Andrés, X, Arnarson, H, Biescas, E, Mazzei, M, Fraisier, C, McNeilly, TN, Liu, C, Perez, M, Carrozza, ML, Bandecchi, P, Solano, C, Crespo, H, Glaria, I, Huard, C, Shaw, DJ, de Blas, I, de Andrés, D, Tolari, F, Rosati, S, Suzan-Monti, M, Andrésdóttir, V, Þorsteinsdóttir, S, Pétursson, G, Lujan, L, Pepin, M, Amorena, B, Blacklaws, B & Harkiss, GD 2008, 'Mucosal immunization against ovine lentivirus using PEI-DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes', Vaccine, vol. 26, no. 35, pp. 4494-4505. https://doi.org/10.1016/j.vaccine.2008.06.065

TY - JOUR

T1 - Mucosal immunization against ovine lentivirus using PEI-DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes

AU - Reina, R.

AU - Barbezange, C.

AU - Niesalla, H.

AU - de Andrés, X.

AU - Arnarson, Hallgrímur

AU - Biescas, E.

AU - Mazzei, M.

AU - Fraisier, C.

AU - McNeilly, T. N.

AU - Liu, C.

AU - Perez, M.

AU - Carrozza, M. L.

AU - Bandecchi, P.

AU - Solano, C.

AU - Crespo, H.

AU - Glaria, I.

AU - Huard, C.

AU - Shaw, D. J.

AU - de Blas, I.

AU - de Andrés, D.

AU - Tolari, F.

AU - Rosati, S.

AU - Suzan-Monti, M.

AU - Andrésdóttir, Valgerður

AU - Þorsteinsdóttir, Sigurbjörg

AU - Pétursson, Gudmundur

AU - Lujan, L.

AU - Pepin, M.

AU - Amorena, B.

AU - Blacklaws, B.

AU - Harkiss, G. D.

N1 - Funding Information: This study was supported by grants from European Union (QLK2-CT-2002-00617) and Spanish CICYT (AGL2003-08977-C03-01 and AGL2006-13410C-06/01/GAN). Ramsés Reina and Ximena de Andrés were supported by a fellowship FPI from the Spanish Ministry of Science and Education. Tom McNeilly was supported by a Ph.D. studentship from the Royal (Dick) College of Veterinary Studies, University of Edinburgh. We thank Margaret Ross, Pauline Love, Paolo Caldato, Paul Tonks, Santiago Becerra, Rosario Puyó, Margherita Profiti, Katia Ricci, Giorgia Tozzini, Angela Wheatley, Sigridur Matthiasdottir, and Paul Wright for technical help.

PY - 2008/8/18

Y1 - 2008/8/18

N2 - Sheep were immunized against Visna/Maedi virus (VMV) gag and/or env genes via the nasopharynx-associated lymphoid tissue (NALT) and lung using polyethylenimine (PEI)-DNA complexes and modified vaccinia Ankara, and challenged with live virus via the lung. env immunization enhanced humoral responses prior to but not after VMV challenge. Systemic T cell proliferative and cytotoxic responses were generally low, with the responses following single gag gene immunization being significantly depressed after challenge. A transient reduction in provirus load in the blood early after challenge was observed following env immunization, whilst the gag gene either alone or in combination with env resulted in significantly elevated provirus loads in lung. However, despite this, a significant reduction in lesion score was observed in animals immunized with the single gag gene at post-mortem. Inclusion of IFN-γ in the immunization mixture in general had no significant effects. The results thus showed that protective effects against VMV-induced lesions can be induced following respiratory immunization with the single gag gene, though this was accompanied by an increased pulmonary provirus load.

AB - Sheep were immunized against Visna/Maedi virus (VMV) gag and/or env genes via the nasopharynx-associated lymphoid tissue (NALT) and lung using polyethylenimine (PEI)-DNA complexes and modified vaccinia Ankara, and challenged with live virus via the lung. env immunization enhanced humoral responses prior to but not after VMV challenge. Systemic T cell proliferative and cytotoxic responses were generally low, with the responses following single gag gene immunization being significantly depressed after challenge. A transient reduction in provirus load in the blood early after challenge was observed following env immunization, whilst the gag gene either alone or in combination with env resulted in significantly elevated provirus loads in lung. However, despite this, a significant reduction in lesion score was observed in animals immunized with the single gag gene at post-mortem. Inclusion of IFN-γ in the immunization mixture in general had no significant effects. The results thus showed that protective effects against VMV-induced lesions can be induced following respiratory immunization with the single gag gene, though this was accompanied by an increased pulmonary provirus load.

KW - DNA vaccination

KW - Lentivirus

KW - Mucosal

KW - Sheep

UR - http://www.scopus.com/inward/record.url?scp=48449092571&partnerID=8YFLogxK

U2 - 10.1016/j.vaccine.2008.06.065

DO - 10.1016/j.vaccine.2008.06.065

M3 - Article

C2 - 18606204

AN - SCOPUS:48449092571

SN - 0264-410X

VL - 26

SP - 4494

EP - 4505

JO - Vaccine

JF - Vaccine

IS - 35

ER -

Mucosal immunization against ovine lentivirus using PEI-DNA complexes and modified vaccinia Ankara encoding the gag and/or env genes

Abstract

Bibliographical note

Other keywords

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