Molecular characterization and phylogenetic analysis of metallothionein gene in Catla Catla

M. A. Rather, I. A. Bhat, N. Sharma, R. Saha, T. Hussain, R. Sharma*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Metallothionein gene (MT) has been used commonly as a potential molecular marker to detect the harmful effects of heavy metals in aquatic environment. Fish(Catla catla) was exposed to sliver nanoparticles for induction of MT gene. Gene MT was successfully characterized and 179bp amplicon product was amplified. MT-2 gene, isolated from the liver, was highly similar to the Cyprinus carpio metallothionein gene. The particle size of the sliver nanoparticles were found to be in the range of 70nm to 585nm with an average size of 192 run. All the above results revealed thatfish MT gene would be a useful biomarker for metal pollution.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalJournal of Industrial Pollution Control
Volume31
Issue number1
Publication statusPublished - 2015

Bibliographical note

Publisher Copyright:
© EM International Printed in India. All rights reserved.

Other keywords

  • Biomarker
  • Metallothionein
  • Nanoparticles
  • Pollution

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