Mitochondrial respiration in human viable platelets--methodology and influence of gender, age and storage.

Fredrik Sjövall, Johannes K H Ehinger, Sigurður Einar Marelsson, Saori Morota, Eleonor Asander Frostner, Hiroyuki Uchino, Johan Lundgren, Einar Arnbjörnsson, Magnus J Hansson, Vineta Fellman, Eskil Elmér

Research output: Contribution to journalArticlepeer-review


Studying whole cell preparations with intact mitochondria and respiratory complexes has a clear benefit compared to isolated or disrupted mitochondria due to the dynamic interplay between mitochondria and other cellular compartments. Platelet mitochondria have a potential to serve as a source of human viable mitochondria when studying mitochondrial physiology and pathogenic mechanisms, as well as for the diagnostics of mitochondrial diseases. The objective of the present study was to perform a detailed evaluation of platelet mitochondrial respiration using high-resolution respirometry. Further, we aimed to explore the limits of sample size and the impact of storage as well as to establish a wide range of reference data from different pediatric and adult cohorts. Our results indicate that platelet mitochondria are well suited for ex-vivo analysis with the need for minute sample amounts and excellent reproducibility and stability.
Original languageEnglish
Publication statusPublished - Jan 2013

Other keywords

  • Adult
  • Age Factors
  • Aged
  • Blood Platelets
  • Cell Respiration
  • Child
  • Child, Preschool
  • Cytological Techniques
  • Female
  • Humans
  • Infant
  • Male
  • Middle Aged
  • Mitochondria
  • Sex Factors
  • Specimen Handling
  • Young Adult


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