Macromolecular interaction and the electrophoretic mobility of esterase-5 from Drosophila pseudoobscura

Einar Árnason*, Geoffrey K. Chambers

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Esterase-5 is one of the most polymorphic loci in Drosophila pseudoobscura. Some variants reportedly produce a dimeric enzyme, while a few produce a monomeric form. This paper reports the finding that during electrophoresis ESTERASE-5 exists in a dynamic equilibrium between monomers and dimers, an equilibrium that is dependent on the running temperature of the gels. This is shown by a series of analytical electrophoresis experiments in which the apparent molecular weights of several variants are determined at four different temperatures. Increasing temperatures result in a linear decrease in the logarithm of apparent molecular weights. Macromolecular interactions thus are a significant determinant of EST-5 electrophoretic mobility.

Original languageEnglish
Pages (from-to)287-307
Number of pages21
JournalBiochemical Genetics
Issue number3-4
Publication statusPublished - Apr 1987

Other keywords

  • Drosophila pseudoobscura
  • electrophoresis
  • esterase-5
  • macromolecular interactions
  • temperature


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