Leucine tRNA family of Escherichia coli: Nucleotide sequence of the supP(Am) suppressor gene

S. Thorbjarnardottir, T. Dingermann, T. Rafnar, O. S. Andrésson, D. Söll, G. Eggertsson

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)

Abstract

We describe the cloning and the DNA sequence of an amber suppressor allele of the Escherichia coli leuX (supP) gene. The suppressor allele codes for a tRNA with anticodon CUA, presumably derived by a single base change from a CAA anticodon. The mature coding sequence of the leuX gene is preceded by a putative Pribnow box sequence (TATAAT) and followed by a termination signal. The sequence of the leuX-coded tRNA is compared with the sequences of the four remaining tRNA(Leu) isoacceptors of E. coli and with two tRNA(Leu) species from bacteriophage T4 and T5. The conserved nucleotides in these seven tRNAs recognized by E. coli leucyl-tRNA synthetase are located mainly in the aminoacyl stem and in the D-stem/loop region.

Original languageEnglish
Pages (from-to)219-222
Number of pages4
JournalJournal of Bacteriology
Volume161
Issue number1
Publication statusPublished - 1985

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