Large scale cultivation of Hydrogenobacter thermophilus on hydrogen

G. O. Hreggvidsson; T. Gustavsson; O. Holst; K. Kronsbein; S. Hjörleifsdottir; T. Skjenstad; J. K. Kristjansson

doi:10.1111/j.1472-765X.1995.tb01059.x

Large scale cultivation of Hydrogenobacter thermophilus on hydrogen

G. O. Hreggvidsson, T. Gustavsson, O. Holst, K. Kronsbein, S. Hjörleifsdottir, T. Skjenstad, J. K. Kristjansson^*

^*Corresponding author for this work

Faculty of Life and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

The thermophilic hydrogen‐oxidizing eubacterium, Hydrogenobacter thermophilus was grown with a semi‐continuous gas feed in a 150 1 fermentor, on different ratios of H₂, O₂ (air) and CO₂ and on media of variable mineral salts composition. The best gas ratio during the exponential phase was approximately 0.5:1.0:0.03 atm of H₂: air: CO₂. Cell densities were increased by increasing the concentration of ammonium and phosphate in the medium. The maximum cell density obtained under the most favourable conditions was 2.62 g wet wt 1^‐1, corresponding to an absorption of 1.6 at 600 nm. The maximum specific growth rate (u) was 0.44 h^‐1.

Original language	English
Pages (from-to)	277-281
Number of pages	5
Journal	Letters in Applied Microbiology
Volume	21
Issue number	5
DOIs	https://doi.org/10.1111/j.1472-765X.1995.tb01059.x
Publication status	Published - Nov 1995

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title = "Large scale cultivation of Hydrogenobacter thermophilus on hydrogen",

abstract = "The thermophilic hydrogen‐oxidizing eubacterium, Hydrogenobacter thermophilus was grown with a semi‐continuous gas feed in a 150 1 fermentor, on different ratios of H2, O2 (air) and CO2 and on media of variable mineral salts composition. The best gas ratio during the exponential phase was approximately 0.5:1.0:0.03 atm of H2: air: CO2. Cell densities were increased by increasing the concentration of ammonium and phosphate in the medium. The maximum cell density obtained under the most favourable conditions was 2.62 g wet wt 1‐1, corresponding to an absorption of 1.6 at 600 nm. The maximum specific growth rate (u) was 0.44 h‐1.",

author = "Hreggvidsson, {G. O.} and T. Gustavsson and O. Holst and K. Kronsbein and S. Hj{\"o}rleifsdottir and T. Skjenstad and Kristjansson, {J. K.}",

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T1 - Large scale cultivation of Hydrogenobacter thermophilus on hydrogen

AU - Hreggvidsson, G. O.

AU - Gustavsson, T.

AU - Holst, O.

AU - Kronsbein, K.

AU - Hjörleifsdottir, S.

AU - Skjenstad, T.

AU - Kristjansson, J. K.

PY - 1995/11

Y1 - 1995/11

N2 - The thermophilic hydrogen‐oxidizing eubacterium, Hydrogenobacter thermophilus was grown with a semi‐continuous gas feed in a 150 1 fermentor, on different ratios of H2, O2 (air) and CO2 and on media of variable mineral salts composition. The best gas ratio during the exponential phase was approximately 0.5:1.0:0.03 atm of H2: air: CO2. Cell densities were increased by increasing the concentration of ammonium and phosphate in the medium. The maximum cell density obtained under the most favourable conditions was 2.62 g wet wt 1‐1, corresponding to an absorption of 1.6 at 600 nm. The maximum specific growth rate (u) was 0.44 h‐1.

AB - The thermophilic hydrogen‐oxidizing eubacterium, Hydrogenobacter thermophilus was grown with a semi‐continuous gas feed in a 150 1 fermentor, on different ratios of H2, O2 (air) and CO2 and on media of variable mineral salts composition. The best gas ratio during the exponential phase was approximately 0.5:1.0:0.03 atm of H2: air: CO2. Cell densities were increased by increasing the concentration of ammonium and phosphate in the medium. The maximum cell density obtained under the most favourable conditions was 2.62 g wet wt 1‐1, corresponding to an absorption of 1.6 at 600 nm. The maximum specific growth rate (u) was 0.44 h‐1.

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Large scale cultivation of Hydrogenobacter thermophilus on hydrogen

Abstract

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