Isolation and characterization of Thermus thermophilus Gy1211 from a deep-sea hydrothermal vent

Viggó Thór Marteinsson; Jean Louis Birrien; Gérard Raguénès; Milton S. Da Costa; Daniel Prieur

doi:10.1007/s007920050123

Isolation and characterization of Thermus thermophilus Gy1211 from a deep-sea hydrothermal vent

Viggó Thór Marteinsson^*, Jean Louis Birrien, Gérard Raguénès, Milton S. Da Costa, Daniel Prieur

^*Corresponding author for this work

Faculty of Food Science and Nutrition

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

We examined a single, non-spore-forming, aerobic, thermophilic strain that was isolated from a deep-sea hydrothermal vent in the Guaymas Basin at a depth of 2000 m and initially placed in a phenetic group with Thermus scotoductus (X-1). We identified this deep-sea isolate as a new strain belonging to Thermus thermophilus using several parameters. DNA-DNA hybridization under stringent conditions showed 74% similarity between the deep-sea isolate and T. thermophilus HB-8(T) (T = type strain). Phenotypic characteristics, such as the utilization of carbon sources, hydrolysis of different compounds, and antibiotic sensitivity were identical in the two strains. The polar lipids composition showed that strain Gy1211 belonged to the genus Thermus. The fatty acids composition indicated that this strain was related to the marine T. thermophilus strain isolated from the Azores. The new isolate T. thermophilus strain Gy1211 grew optimally at 75°C, pH 8.0, and 2% NaCl. A hydrostatic pressure of 20MPa, similar to the in situ hydrostatic pressure of the deep-sea vent from which the strain was isolated, had no effect on growth. Strain HB-8(T), however, showed slower growth under these conditions.

Original language	English
Pages (from-to)	247-251
Number of pages	5
Journal	Extremophiles
Volume	3
Issue number	4
DOIs	https://doi.org/10.1007/s007920050123
Publication status	Published - Nov 1999

Bibliographical note

Funding Information:
Acknowledgments We thank Anne-Marie Alayse, chief scientist of the cruise “Guaynaut”, for inviting one of us to participate in that cruise. Thanks are also due to Anne Donval and Gérard Sinquin for the electron microscopy Viggó Thór Marteinsson was supported by a French-Icelandic fellowship (Programme Franco-Islandais). This work was supported by GDR 1006 CNRS/Ifremer, the CPER 94–95 (Contrat de Plan Etat-Région), the European Union (Feder, objectif 5b), and Conseil Général du Finistèr.

Other keywords

Hydrothermal vents
Marine
Taxonomy
Thermophiles
Thermus thermophilus

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10.1007/s007920050123

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@article{370117636adb405f9f79174bfacbec75,

title = "Isolation and characterization of Thermus thermophilus Gy1211 from a deep-sea hydrothermal vent",

abstract = "We examined a single, non-spore-forming, aerobic, thermophilic strain that was isolated from a deep-sea hydrothermal vent in the Guaymas Basin at a depth of 2000 m and initially placed in a phenetic group with Thermus scotoductus (X-1). We identified this deep-sea isolate as a new strain belonging to Thermus thermophilus using several parameters. DNA-DNA hybridization under stringent conditions showed 74% similarity between the deep-sea isolate and T. thermophilus HB-8(T) (T = type strain). Phenotypic characteristics, such as the utilization of carbon sources, hydrolysis of different compounds, and antibiotic sensitivity were identical in the two strains. The polar lipids composition showed that strain Gy1211 belonged to the genus Thermus. The fatty acids composition indicated that this strain was related to the marine T. thermophilus strain isolated from the Azores. The new isolate T. thermophilus strain Gy1211 grew optimally at 75°C, pH 8.0, and 2% NaCl. A hydrostatic pressure of 20MPa, similar to the in situ hydrostatic pressure of the deep-sea vent from which the strain was isolated, had no effect on growth. Strain HB-8(T), however, showed slower growth under these conditions.",

keywords = "Hydrothermal vents, Marine, Taxonomy, Thermophiles, Thermus thermophilus",

author = "Marteinsson, {Vigg{\'o} Th{\'o}r} and Birrien, {Jean Louis} and G{\'e}rard Ragu{\'e}n{\`e}s and {Da Costa}, {Milton S.} and Daniel Prieur",

note = "Funding Information: Acknowledgments We thank Anne-Marie Alayse, chief scientist of the cruise “Guaynaut”, for inviting one of us to participate in that cruise. Thanks are also due to Anne Donval and G{\'e}rard Sinquin for the electron microscopy Vigg{\'o} Th{\'o}r Marteinsson was supported by a French-Icelandic fellowship (Programme Franco-Islandais). This work was supported by GDR 1006 CNRS/Ifremer, the CPER 94–95 (Contrat de Plan Etat-R{\'e}gion), the European Union (Feder, objectif 5b), and Conseil G{\'e}n{\'e}ral du Finist{\`e}r.",

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AU - Da Costa, Milton S.

AU - Prieur, Daniel

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N2 - We examined a single, non-spore-forming, aerobic, thermophilic strain that was isolated from a deep-sea hydrothermal vent in the Guaymas Basin at a depth of 2000 m and initially placed in a phenetic group with Thermus scotoductus (X-1). We identified this deep-sea isolate as a new strain belonging to Thermus thermophilus using several parameters. DNA-DNA hybridization under stringent conditions showed 74% similarity between the deep-sea isolate and T. thermophilus HB-8(T) (T = type strain). Phenotypic characteristics, such as the utilization of carbon sources, hydrolysis of different compounds, and antibiotic sensitivity were identical in the two strains. The polar lipids composition showed that strain Gy1211 belonged to the genus Thermus. The fatty acids composition indicated that this strain was related to the marine T. thermophilus strain isolated from the Azores. The new isolate T. thermophilus strain Gy1211 grew optimally at 75°C, pH 8.0, and 2% NaCl. A hydrostatic pressure of 20MPa, similar to the in situ hydrostatic pressure of the deep-sea vent from which the strain was isolated, had no effect on growth. Strain HB-8(T), however, showed slower growth under these conditions.

AB - We examined a single, non-spore-forming, aerobic, thermophilic strain that was isolated from a deep-sea hydrothermal vent in the Guaymas Basin at a depth of 2000 m and initially placed in a phenetic group with Thermus scotoductus (X-1). We identified this deep-sea isolate as a new strain belonging to Thermus thermophilus using several parameters. DNA-DNA hybridization under stringent conditions showed 74% similarity between the deep-sea isolate and T. thermophilus HB-8(T) (T = type strain). Phenotypic characteristics, such as the utilization of carbon sources, hydrolysis of different compounds, and antibiotic sensitivity were identical in the two strains. The polar lipids composition showed that strain Gy1211 belonged to the genus Thermus. The fatty acids composition indicated that this strain was related to the marine T. thermophilus strain isolated from the Azores. The new isolate T. thermophilus strain Gy1211 grew optimally at 75°C, pH 8.0, and 2% NaCl. A hydrostatic pressure of 20MPa, similar to the in situ hydrostatic pressure of the deep-sea vent from which the strain was isolated, had no effect on growth. Strain HB-8(T), however, showed slower growth under these conditions.

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KW - Taxonomy

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ER -

Isolation and characterization of Thermus thermophilus Gy1211 from a deep-sea hydrothermal vent

Abstract

Bibliographical note

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