Isolation and characterization of lambda transducing phages for the E. coli genes ksgA and pdxA

Ólafur Sigmar Andrésson*, Julian E. Davies

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Lambda phages carrying the Escherichia coli genes ksgA and pdxA were isolated from secondary site lysogens in araB. 1) The phage genomes were characterized by genetic complementation tests, restriction endonuclease digestion and electron microscopy. 2) A 6.3 kilobasepair (kb) EcoRI restriction fragment carrying both ksgA and pdxA was cloned in a lambda vector; this fragment has proven useful in further characterization of the ksgA gene (Andrésson and Davies, 1980a, b). The ksgA and pdxA genes are about 14 and 12-13 kb, respectively, counterclockwise of the arabinose operon and 1.5 and 2.5-3.5 kb clockwise of folA.

Original languageEnglish
Pages (from-to)201-209
Number of pages9
JournalMGG Molecular & General Genetics
Volume179
Issue number1
DOIs
Publication statusPublished - Aug 1980

Bibliographical note

Copyright © 1969, Springer-Verlag

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