Inhibition of T cell activation by the extracellular matrix protein tenascin

Timothy J. Hemesath, Linda S. Marton, Kari Stefansson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Tenascin (TN) is an extracellular matrix protein that is expressed widely in the fetus and sparingly in the adult, but reappears at high levels in certain areas of tissue insult such as tumor matrices and sites of wound healing. We show here that soluble TN inhibits proliferation of human T cells in response to αCD3 Ab co-immobilized with the extracellular matrix protein fibronectin (FN). TN also inhibits proliferation driven by αCD3/IL-2 or by phorbol ester/IL-2, and it prevents high level induction of IL-2R. The presence of TN in culture medium does not detectably alter the pattern of tyrosine phosphorylation resulting from T cell triggering with αCD3, but at later time points prevents the appearance of functional NF-AT1 transcription factor complexes in T cell nuclear extracts. These findings are consistent with the postulated role for TN as a natural antagonist to FN action, and suggest that T cell responses occurring at tissue sites in which TN is expressed could be influenced by its presence.

Original languageEnglish
Pages (from-to)5199-5207
Number of pages9
JournalJournal of Immunology
Volume152
Issue number11
Publication statusPublished - 1 Jun 1994

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