TY - JOUR
T1 - Immunomodulatory and Antimicrobial potential of ethanolic extract of Himalayan Myrica esculanta in Oncorhynchus mykiss
T2 - Molecular modelling with Aeromonas hydrophila functional proteins
AU - Bhat, Raja Aadil Hussain
AU - Rehman, Sabeehah
AU - Tandel, Ritesh Shantilal
AU - Dash, Pragyan
AU - Bhandari, Ankita
AU - Ganie, Parvaiz Ahmad
AU - Shah, Tarang K.
AU - Pant, Kushagra
AU - Yousuf, Dar Jaffer
AU - Bhat, Irfan Ahmad
AU - Chandra, Suresh
AU - Mallik, Sumanta K.
AU - Sarma, Debajit
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2/1
Y1 - 2021/2/1
N2 - The present study was carried out to investigate the antimicrobial and immunomodulatory activity of Myrica esculenta ethanolic leaf extract (MeALE) in rainbow trout. The juvenile fish were immersed in 20 mg/l (T1) and 40 mg/l (T2) MeALE for 2 and 6 h and then challenged with pathogenic bacteria, A. hydrophila (ATCC 7966, USA) after 6 h post immersion in MeALE. The immuno-haematological parameters and relative percentage survival were recorded over 7 days post-exposure. The amino acid interaction of functional proteins of bacteria with myricetin, the active ingredient of M. esculenta was performed by AutoDock Vina software. FTIR detected the characteristic peak values and revealed the presence of various functional groups, viz. alkenes, amines, alcohols, phenols and aromatic compounds. The MIC of MeALE was found to be 2 mg/ml while MBC was recorded to be 25 mg/ml. Molecular docking revealed a strong affinity between myricetin and functional proteins of the pathogen. The fish immersed in 40 mg/l MeALE showed a significant increase in haematological indices and non-specific immunological parameters. The current study reports the successful use of MeALE as an antimicrobial and immunostimulatory agent in rainbow trout and recommends the dosage of 40 mg/l MeALE for preventing Aeromonas infection.
AB - The present study was carried out to investigate the antimicrobial and immunomodulatory activity of Myrica esculenta ethanolic leaf extract (MeALE) in rainbow trout. The juvenile fish were immersed in 20 mg/l (T1) and 40 mg/l (T2) MeALE for 2 and 6 h and then challenged with pathogenic bacteria, A. hydrophila (ATCC 7966, USA) after 6 h post immersion in MeALE. The immuno-haematological parameters and relative percentage survival were recorded over 7 days post-exposure. The amino acid interaction of functional proteins of bacteria with myricetin, the active ingredient of M. esculenta was performed by AutoDock Vina software. FTIR detected the characteristic peak values and revealed the presence of various functional groups, viz. alkenes, amines, alcohols, phenols and aromatic compounds. The MIC of MeALE was found to be 2 mg/ml while MBC was recorded to be 25 mg/ml. Molecular docking revealed a strong affinity between myricetin and functional proteins of the pathogen. The fish immersed in 40 mg/l MeALE showed a significant increase in haematological indices and non-specific immunological parameters. The current study reports the successful use of MeALE as an antimicrobial and immunostimulatory agent in rainbow trout and recommends the dosage of 40 mg/l MeALE for preventing Aeromonas infection.
KW - Aeromonas hydrophila
KW - Antibiotics
KW - Aquaculture
KW - Immunological parameters
KW - Myrica esculenta
KW - Rainbow trout
UR - http://www.scopus.com/inward/record.url?scp=85097785612&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2020.736213
DO - 10.1016/j.aquaculture.2020.736213
M3 - Article
AN - SCOPUS:85097785612
SN - 0044-8486
VL - 533
JO - Aquaculture
JF - Aquaculture
M1 - 736213
ER -