Immunochemical characterization of charge isomers of bacteria-derived human growth hormone with monoclonal antibodies

Ingileif Jónsdóttir; Hans Peter T. Ekre; Bo Skoog; Peter Perlmann

doi:10.1016/0014-5793(84)80823-6

Immunochemical characterization of charge isomers of bacteria-derived human growth hormone with monoclonal antibodies

Ingileif Jónsdóttir^*, Hans Peter T. Ekre, Bo Skoog, Peter Perlmann

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Monoclonal antibodies were used to study the immunochemical nature of charge isomers of bacterially produced methionyl human growth hormone. After isoelectric focusing of the hormone the 12 monoclonal antibodies reacted similarly in immunoblotting experiments and none of them could discriminate between the two isolated charge isomers in ELISA. This indicates that the generation of charge isomers of met-hGH does not result is loss of the determinants recognized by the monoclonal antibodies and that the conformation of the two main charge isomers is identical within these determinants.

Original language	English
Pages (from-to)	15-18
Number of pages	4
Journal	FEBS Letters
Volume	167
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(84)80823-6
Publication status	Published - 13 Feb 1984

Bibliographical note

Funding Information:
We thank Mr. Rahul Ghosh for excellent technical assistanceD, r Klavs Berzins, Dept. of Immunology, University of Stockholm, for valuablea dvicec oncerningt he immunoblottinge x-periments,a nd Dr Linda Fryklund, Researcha nd Development,K abiVitrum AB, Stockholm, for readingt he manuscript.T his work was supported by a grant from KabiVitrum AB.

Other keywords

Charge isomer
ELISA
Human growth hormone
Immunoblotting
Monoclonal antibody

Access to Document

10.1016/0014-5793(84)80823-6

Cite this

@article{d96ace6606294bcb8c740c9004c519b3,

title = "Immunochemical characterization of charge isomers of bacteria-derived human growth hormone with monoclonal antibodies",

abstract = "Monoclonal antibodies were used to study the immunochemical nature of charge isomers of bacterially produced methionyl human growth hormone. After isoelectric focusing of the hormone the 12 monoclonal antibodies reacted similarly in immunoblotting experiments and none of them could discriminate between the two isolated charge isomers in ELISA. This indicates that the generation of charge isomers of met-hGH does not result is loss of the determinants recognized by the monoclonal antibodies and that the conformation of the two main charge isomers is identical within these determinants.",

keywords = "Charge isomer, ELISA, Human growth hormone, Immunoblotting, Monoclonal antibody",

author = "Ingileif J{\'o}nsd{\'o}ttir and Ekre, {Hans Peter T.} and Bo Skoog and Peter Perlmann",

note = "Funding Information: We thank Mr. Rahul Ghosh for excellent technical assistanceD, r Klavs Berzins, Dept. of Immunology, University of Stockholm, for valuablea dvicec oncerningt he immunoblottinge x-periments,a nd Dr Linda Fryklund, Researcha nd Development,K abiVitrum AB, Stockholm, for readingt he manuscript.T his work was supported by a grant from KabiVitrum AB.",

year = "1984",

month = feb,

day = "13",

doi = "10.1016/0014-5793(84)80823-6",

language = "English",

volume = "167",

pages = "15--18",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Wiley-Blackwell",

number = "1",

}

TY - JOUR

T1 - Immunochemical characterization of charge isomers of bacteria-derived human growth hormone with monoclonal antibodies

AU - Jónsdóttir, Ingileif

AU - Ekre, Hans Peter T.

AU - Skoog, Bo

AU - Perlmann, Peter

N1 - Funding Information: We thank Mr. Rahul Ghosh for excellent technical assistanceD, r Klavs Berzins, Dept. of Immunology, University of Stockholm, for valuablea dvicec oncerningt he immunoblottinge x-periments,a nd Dr Linda Fryklund, Researcha nd Development,K abiVitrum AB, Stockholm, for readingt he manuscript.T his work was supported by a grant from KabiVitrum AB.

PY - 1984/2/13

Y1 - 1984/2/13

N2 - Monoclonal antibodies were used to study the immunochemical nature of charge isomers of bacterially produced methionyl human growth hormone. After isoelectric focusing of the hormone the 12 monoclonal antibodies reacted similarly in immunoblotting experiments and none of them could discriminate between the two isolated charge isomers in ELISA. This indicates that the generation of charge isomers of met-hGH does not result is loss of the determinants recognized by the monoclonal antibodies and that the conformation of the two main charge isomers is identical within these determinants.

AB - Monoclonal antibodies were used to study the immunochemical nature of charge isomers of bacterially produced methionyl human growth hormone. After isoelectric focusing of the hormone the 12 monoclonal antibodies reacted similarly in immunoblotting experiments and none of them could discriminate between the two isolated charge isomers in ELISA. This indicates that the generation of charge isomers of met-hGH does not result is loss of the determinants recognized by the monoclonal antibodies and that the conformation of the two main charge isomers is identical within these determinants.

KW - Charge isomer

KW - ELISA

KW - Human growth hormone

KW - Immunoblotting

KW - Monoclonal antibody

UR - http://www.scopus.com/inward/record.url?scp=0021340949&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(84)80823-6

DO - 10.1016/0014-5793(84)80823-6

M3 - Article

C2 - 6698201

AN - SCOPUS:0021340949

VL - 167

SP - 15

EP - 18

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 1

ER -

Immunochemical characterization of charge isomers of bacteria-derived human growth hormone with monoclonal antibodies

Abstract

Bibliographical note

Other keywords

Access to Document

Fingerprint

Cite this