Immunochemical characterization of charge isomers of bacteria-derived human growth hormone with monoclonal antibodies

Ingileif Jónsdóttir*, Hans Peter T. Ekre, Bo Skoog, Peter Perlmann

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Monoclonal antibodies were used to study the immunochemical nature of charge isomers of bacterially produced methionyl human growth hormone. After isoelectric focusing of the hormone the 12 monoclonal antibodies reacted similarly in immunoblotting experiments and none of them could discriminate between the two isolated charge isomers in ELISA. This indicates that the generation of charge isomers of met-hGH does not result is loss of the determinants recognized by the monoclonal antibodies and that the conformation of the two main charge isomers is identical within these determinants.

Original languageEnglish
Pages (from-to)15-18
Number of pages4
JournalFEBS Letters
Volume167
Issue number1
DOIs
Publication statusPublished - 13 Feb 1984

Bibliographical note

Funding Information:
We thank Mr. Rahul Ghosh for excellent technical assistanceD, r Klavs Berzins, Dept. of Immunology, University of Stockholm, for valuablea dvicec oncerningt he immunoblottinge x-periments,a nd Dr Linda Fryklund, Researcha nd Development,K abiVitrum AB, Stockholm, for readingt he manuscript.T his work was supported by a grant from KabiVitrum AB.

Other keywords

  • Charge isomer
  • ELISA
  • Human growth hormone
  • Immunoblotting
  • Monoclonal antibody

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