Hyporesponsiveness following booster immunization with bacterial polysaccharides is caused by apoptosis of memory B cells

Siggeir F. Brynjolfsson, Maren Henneken, Stefania P. Bjarnarson, Elena Mori, Giuseppe Del Giudice, Ingileif Jonsdottir

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

Background. Repeated immunizations with polysaccharide (PS) vaccines cause hyporesponsiveness through undefined mechanisms. We assessed the effects of a PS booster on immune responses, frequency, and survival of PS-specific B-cell subpopulations in spleen and bone marrow. Methods. Neonatal mice were primed with meningococcus serotype C (MenC) conjugate MenC-CRM 197+CpG1826, boosted with MenC-CRM 197, MenC-PS, or saline; subsequently, bromodeoxyuridine (BrdU) was injected daily intraperitoneally. MenC-PS-specific cells were labeled with fluorescent MenC-PS and phenotyped by flow cytometry. Results. After MenC-PS booster, proliferating (BrdU +) MenC-PS-specific naive B cells (CD138 -/B220 +; P =. 0003) and plasma cells (CD138 +/B220 -; P =. 0002) in spleen were fewer than after saline booster. BrdU + MenC-PS-specific plasma cells were also reduced in bone marrow (P =. 0308). Compared to saline, MenC-PS booster reduced BrdU + IgG + MenC-PS-specific B cells in spleen (P =. 0002). Twelve hours after the MenC-PS booster, an increased frequency of apoptotic (AnnexinV +) MenC-PS-specific B cells in spleen was observed compared with MenC-CRM197 (P =. 0286) or saline (P =. 001) boosters.Conclusions.We demonstrated that the MenC-PS booster significantly reduced the frequency of newly activated MenC-PS-specific B cells - mostly switched IgG + memory cells - by driving them into apoptosis. It shows directly that apoptosis of PS-specific memory cells is the cause of PS-induced hyporesponsiveness. These results should be taken into account prior to consideration of the use of PS vaccines.

Original languageEnglish
Pages (from-to)422-430
Number of pages9
JournalJournal of Infectious Diseases
Volume205
Issue number3
DOIs
Publication statusPublished - 1 Feb 2012

Bibliographical note

Funding Information:
Financial support. This work was funded by The University of Iceland Research Fund and the Landspitali University Hospital Research Fund.

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