High molecular weight growth hormone (>160 kd) in human serum characterized with monoclonal antibodies

Ingileif Jónsdóttir, Marguerite Luthman*, Hans Peter T. Ekre, Bo Skoog, Peter Brostedt, Paul Roos, Sigbritt Werner, Peter Perlmann

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Human growth hormone (hGH) was analyzed by six monoclonal antibodies (Mabs) and a polyclonal antiserum (Pas) before and after molecular sieve chromatography of sera from healthy subjects. Their hGH levels were between <0.2 and 0.4 ng/ml as determined with Pas. The six Mabs reacted with five distinct epitopes and bound to a hGH fragment corresponding to the amino acid sequence 15-125. Two of the Mabs showed reduced binding to 20-kD hGH. The binding of Mabs to dimeric forms of hGH varied. Human GH levels in unfractionated sera as determined with Mabs were <3.1-390 ng/ml. After molecular sieve chromatography of the sera, one peak of hGH-immuno- reactive material of high molecular weight (>160 kD) and one at the elution volume of monomeric hGH were determined with Pas and Mabs. The major part of the high molecular weight hGH (>160 kD) seemed to consist of 22-kD hGH molecules, since Pas and all Mabs detected the hGH immunoreactivity (>160 kD) in a similar manner. This high molecular weight hGH (>160 kD) was distinguishable from the identified, receptor-like hGH-binding protein in serum.

Original languageEnglish
Pages (from-to)197-204
Number of pages8
JournalHormone Research in Paediatrics
Volume41
Issue number5-6
DOIs
Publication statusPublished - 1994

Other keywords

  • Growth hormone, human, high molecular weight
  • Heterogeneity, hGH
  • Monoclonal antibodies

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