TY - JOUR
T1 - Glucosamine increases the expression of YKL-40 and osteogenic marker genes in hMSC during osteogenic differentiation
AU - Lieder, Ramona
AU - Reynisdóttir, Sigríður Þóra
AU - Thormódsson, Finnbogi
AU - Ng, Chuen How
AU - Einarsson, Jon Magnús
AU - Gíslason, Jóhannes
AU - Björnsson, Jóhannes
AU - Gudmundsson, Sveinn
AU - Petersen, Pétur Henry
AU - Sigurjónsson, Ólafur Eysteinn
N1 - Publisher Copyright:
© 2012, The Author(s).
PY - 2012/4/1
Y1 - 2012/4/1
N2 - Human mesenchymal stem cells (hMSC) can be expanded in vitro and differentiated towards osteogenic, chondrogenic or adipogenic lineages, making them an attractive source for tissue engineering and regenerative medicine. Chitinase-like-proteins (CLPs) belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling. The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC. Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC. YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation. A slight but nonsignificant increase in YKL-40 expression was observed with glucosamine, accompanied by a pH-dependent delay in mineralization. However, glucosamine induced higher expression of osteogenic marker genes. [InlineMediaObject not available: see fulltext.].
AB - Human mesenchymal stem cells (hMSC) can be expanded in vitro and differentiated towards osteogenic, chondrogenic or adipogenic lineages, making them an attractive source for tissue engineering and regenerative medicine. Chitinase-like-proteins (CLPs) belong to the family 18 glycosyl hydrolases and are believed to play a role in inflammation and tissue remodelling. The aim of this study was to determine the effect of the aminosugar glucosamine on the expression of the CLP YKL-40 during osteogenic differentiation of hMSC. Glucosamine did not affect multipotency of hMSC nor proliferation rate of undifferentiated hMSC. YKL-40 was expressed during both expansion of undifferentiated hMSC and during osteogenic differentiation. A slight but nonsignificant increase in YKL-40 expression was observed with glucosamine, accompanied by a pH-dependent delay in mineralization. However, glucosamine induced higher expression of osteogenic marker genes. [InlineMediaObject not available: see fulltext.].
KW - chitinase-like-protein
KW - mesenchymal stem cells
KW - osteogenic differentiation
KW - YKL-40
UR - http://www.scopus.com/inward/record.url?scp=84871378881&partnerID=8YFLogxK
U2 - 10.1007/s13659-012-0017-0
DO - 10.1007/s13659-012-0017-0
M3 - Article
AN - SCOPUS:84871378881
SN - 2192-2195
VL - 2
SP - 87
EP - 91
JO - Natural Products and Bioprospecting
JF - Natural Products and Bioprospecting
IS - 2
ER -