Abstract
The pan-tropical sea urchin Tripneustes gratilla is an ecologically and economically important shallow water algal grazer. The aquaculture of T. gratilla has spurred growing interest in the population biology of the species, and by extension the generation of more molecular resources. To this purpose, de novo transcriptomes of T. gratilla were generated for two adults, a male and a female, as well as for a cohort of approximately 1000 plutei larvae. Gene expression profiles of three adult tissue samples were quantified and compared. These samples were of gonadal tissue, the neural ring, and pooled tube feet and pedicellariae. Levels of shared and different gene expression between sexes, as well as across functional categories of interest, including the immune system, toxins, genes involved in fertilization, and sensory genes are highlighted. Differences in expression of isoforms between the sexes and Sex determining Region Y-related High Mobility Group box groups is observed. Additionally an expansion of the tumor suppressor DMBT1 is observed in T. gratilla when compared to the annotated genome of the sea urchin Strongylocentrotus purpuratus. The draft transcriptome of T. gratilla is presented here in order to facilitate more genomic level analysis of emerging model sea urchin systems.
| Original language | English |
|---|---|
| Pages (from-to) | 12-18 |
| Number of pages | 7 |
| Journal | Marine Genomics |
| Volume | 41 |
| DOIs | |
| Publication status | Published - Oct 2018 |
Bibliographical note
Funding Information:This research was funded and made possible by the Jessie D. Kay Memorial Fellowship; the Elizabeth A. Kay Endowed Award; the Charles H. & Margaret B. Edmondson Research Fund; the Watson T. Yoshimoto Fellowship, and the Hampton & Meredith Carson Fellowship, both administered by the Ecology, Evolution, and Conservation Biology specialization program at the University of Hawaiʻi at Mānoa . Thanks to Victoria Sindorf, for helpful comments and suggestions. Special thanks to David Cohen and staff of the Ānuenue Fisheries Research Center (DLNR), and to Amy Eggers, of the Hawaiʻi Institute of Marine Biology Genetics Core. Finally, the authors would like to thank two anonymous reviewers for their constructive feedback and insights.
Funding Information:
This research was funded and made possible by the Jessie D. Kay Memorial Fellowship; the Elizabeth A. Kay Endowed Award; the Charles H. & Margaret B. Edmondson Research Fund; the Watson T. Yoshimoto Fellowship, and the Hampton & Meredith Carson Fellowship, both administered by the Ecology, Evolution, and Conservation Biology specialization program at the University of Hawai?i at M?noa. Thanks to Victoria Sindorf, for helpful comments and suggestions. Special thanks to David Cohen and staff of the ?nuenue Fisheries Research Center (DLNR), and to Amy Eggers, of the Hawai?i Institute of Marine Biology Genetics Core. Finally, the authors would like to thank two anonymous reviewers for their constructive feedback and insights.
Funding Information:
This research was funded and made possible by the Jessie D. Kay Memorial Fellowship; the Elizabeth A. Kay Endowed Award; the Charles H. & Margaret B. Edmondson Research Fund; the Watson T. Yoshimoto Fellowship, and the Hampton & Meredith Carson Fellowship, both administered by the Ecology, Evolution, and Conservation Biology specialization program at the University of Hawaiʻi at Mānoa. Thanks to Victoria Sindorf, for helpful comments and suggestions. Special thanks to David Cohen and staff of the Ānuenue Fisheries Research Center (DLNR), and to Amy Eggers, of the Hawaiʻi Institute of Marine Biology Genetics Core. Finally, the authors would like to thank two anonymous reviewers for their constructive feedback and insights.
Publisher Copyright:
© 2018 Elsevier B.V.
Other keywords
- Echinodermata
- Larval expression
- Tissue expression
- Transcriptome