Examination of serum and bovine serum albumin as shear protective agents in agitated cultures of hybridoma cells

Sadettin S. Ozturk, Bernhard O. Palsson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

A murine hybridoma cell line (167.4G5.3) was cultured in batch mode using IMDM containing different serum concentrations and bovine serum albumin (BSA). Cell growth and death, metabolism and antibody production were studied in these cultures. The cells were more susceptible to shear in the stationary and in the decline phase of growth as evidenced by higher death rates. Cell growth was best at high serum concentrations with high specific growth and low specific death rates. When BSA was used instead of serum in IMDM, no protective effect was observed. Cell metabolism and monoclonal antibody production rates were not influenced by the level of serum or by BSA. The use of serum in commercial serum-free media (OPTI-MEM) also resulted in no change in both growth and death rates.

Original languageEnglish
Pages (from-to)13-28
Number of pages16
JournalJournal of Biotechnology
Volume18
Issue number1-2
DOIs
Publication statusPublished - Apr 1991

Bibliographical note

Funding Information:
This work was supported by National Science Foundation Grant No. EET-8712756. The authors thank Dr. J. Latham Claflin for providing the hybridoma cell line used in this study.

Other keywords

  • Agitation
  • Albumin
  • Cell culture
  • Hybridoma
  • Serum
  • Shear

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