TY - JOUR
T1 - Evaluation of a semi-nested PCR for detection of Renibacterium salmoninarum in samples from kidney, gill and ovarian fluid of Atlantic salmon broodfish
AU - Arnason, Ivar Örn
AU - Sigurdardottir, Sunna
AU - Kristmundsson, Arni
AU - Svansson, Vilhjalmur
AU - Gudmundsdottir, Sigridur
PY - 2013
Y1 - 2013
N2 - A semi-nested PCR (snPCR) for detection of Renibacterium salmoninarum that causes bacterial kidney disease (BKD) in salmonids was constructed. The efficacy of the snPCR was evaluated by comparison with nested PCR (nPCR) and two ELISA methods on kidney, ovarian fluid and gill samples collected from Atlantic salmon broodfish with escalating disease. The PCR methods using a conventional isolation kit identified equal numbers of positive samples, or 30%, with acceptable agreement. The ratio of positive kidney samples in PCR increased significantly, 42.5% in snPCR and 45% in nPCR, when an FTA minicard was used for DNA isolation. ELISA, using polyclonal antibodies, detected the highest number of positive samples (65%) and ELISA using monoclonal antibodies the lowest (17.5%). Ovarian fluid and gills gave inadequate results and cannot replace kidney samples for determination of R. salmoninarum infection in Atlantic salmon broodfish.
AB - A semi-nested PCR (snPCR) for detection of Renibacterium salmoninarum that causes bacterial kidney disease (BKD) in salmonids was constructed. The efficacy of the snPCR was evaluated by comparison with nested PCR (nPCR) and two ELISA methods on kidney, ovarian fluid and gill samples collected from Atlantic salmon broodfish with escalating disease. The PCR methods using a conventional isolation kit identified equal numbers of positive samples, or 30%, with acceptable agreement. The ratio of positive kidney samples in PCR increased significantly, 42.5% in snPCR and 45% in nPCR, when an FTA minicard was used for DNA isolation. ELISA, using polyclonal antibodies, detected the highest number of positive samples (65%) and ELISA using monoclonal antibodies the lowest (17.5%). Ovarian fluid and gills gave inadequate results and cannot replace kidney samples for determination of R. salmoninarum infection in Atlantic salmon broodfish.
KW - Atlantic salmon
KW - Bacterial kidney Disease
KW - ELISA
KW - Nested PCR
KW - Renibacterium salmoninarum
KW - Semi-nested PCR
UR - http://www.scopus.com/inward/record.url?scp=84884549938&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84884549938
SN - 1670-567X
VL - 26
SP - 49
EP - 57
JO - Icelandic Agricultural Sciences
JF - Icelandic Agricultural Sciences
IS - 1
ER -