Enzyme-linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides

Catherine M. Wernette; Carl E. Frasch; Dace Madore; George Carlone; David Goldblatt; Brian Plikaytis; William Benjamin; Sally A. Quataert; Steve Hildreth; Daniel J. Sikkema; Helena Käyhty; Ingileif Jonsdottir; Moon H. Nahm

doi:10.1128/CDLI.10.4.514-519.2003

Enzyme-linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides

Catherine M. Wernette, Carl E. Frasch, Dace Madore, George Carlone, David Goldblatt, Brian Plikaytis, William Benjamin, Sally A. Quataert, Steve Hildreth, Daniel J. Sikkema, Helena Käyhty, Ingileif Jonsdottir, Moon H. Nahm^*

^*Corresponding author for this work

Research output: Contribution to journal › Short survey › peer-review

269 Citations (Scopus)

Abstract

The pneumococcal PS ELISA has a long and complex history. While the procedure has undergone many refinements over the years, we present a current consensus guidance protocol. This protocol is a web-based document for those who want to use the ELISA to test the immune response to pneumococcal PS and PS-protein conjugate vaccines (http://www .vaccine.uab.edu). It contains detailed information on the materials, reagents, conditions, and procedures to aid those interested in implementing the pneumococcal ELISA that yields results that are directly comparable within and between laboratories.

Original language	English
Pages (from-to)	514-519
Number of pages	6
Journal	Clinical and Diagnostic Laboratory Immunology
Volume	10
Issue number	4
DOIs	https://doi.org/10.1128/CDLI.10.4.514-519.2003
Publication status	Published - Jul 2003

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Wernette, C. M., Frasch, C. E., Madore, D., Carlone, G., Goldblatt, D., Plikaytis, B., Benjamin, W., Quataert, S. A., Hildreth, S., Sikkema, D. J., Käyhty, H., Jonsdottir, I., & Nahm, M. H. (2003). Enzyme-linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides. Clinical and Diagnostic Laboratory Immunology, 10(4), 514-519. https://doi.org/10.1128/CDLI.10.4.514-519.2003

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abstract = "The pneumococcal PS ELISA has a long and complex history. While the procedure has undergone many refinements over the years, we present a current consensus guidance protocol. This protocol is a web-based document for those who want to use the ELISA to test the immune response to pneumococcal PS and PS-protein conjugate vaccines (http://www .vaccine.uab.edu). It contains detailed information on the materials, reagents, conditions, and procedures to aid those interested in implementing the pneumococcal ELISA that yields results that are directly comparable within and between laboratories.",

author = "Wernette, {Catherine M.} and Frasch, {Carl E.} and Dace Madore and George Carlone and David Goldblatt and Brian Plikaytis and William Benjamin and Quataert, {Sally A.} and Steve Hildreth and Sikkema, {Daniel J.} and Helena K{\"a}yhty and Ingileif Jonsdottir and Nahm, {Moon H.}",

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doi = "10.1128/CDLI.10.4.514-519.2003",

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Wernette, CM, Frasch, CE, Madore, D, Carlone, G, Goldblatt, D, Plikaytis, B, Benjamin, W, Quataert, SA, Hildreth, S, Sikkema, DJ, Käyhty, H, Jonsdottir, I & Nahm, MH 2003, 'Enzyme-linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides', Clinical and Diagnostic Laboratory Immunology, vol. 10, no. 4, pp. 514-519. https://doi.org/10.1128/CDLI.10.4.514-519.2003

TY - JOUR

T1 - Enzyme-linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides

AU - Wernette, Catherine M.

AU - Frasch, Carl E.

AU - Madore, Dace

AU - Carlone, George

AU - Goldblatt, David

AU - Plikaytis, Brian

AU - Benjamin, William

AU - Quataert, Sally A.

AU - Hildreth, Steve

AU - Sikkema, Daniel J.

AU - Käyhty, Helena

AU - Jonsdottir, Ingileif

AU - Nahm, Moon H.

PY - 2003/7

Y1 - 2003/7

N2 - The pneumococcal PS ELISA has a long and complex history. While the procedure has undergone many refinements over the years, we present a current consensus guidance protocol. This protocol is a web-based document for those who want to use the ELISA to test the immune response to pneumococcal PS and PS-protein conjugate vaccines (http://www .vaccine.uab.edu). It contains detailed information on the materials, reagents, conditions, and procedures to aid those interested in implementing the pneumococcal ELISA that yields results that are directly comparable within and between laboratories.

AB - The pneumococcal PS ELISA has a long and complex history. While the procedure has undergone many refinements over the years, we present a current consensus guidance protocol. This protocol is a web-based document for those who want to use the ELISA to test the immune response to pneumococcal PS and PS-protein conjugate vaccines (http://www .vaccine.uab.edu). It contains detailed information on the materials, reagents, conditions, and procedures to aid those interested in implementing the pneumococcal ELISA that yields results that are directly comparable within and between laboratories.

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C2 - 12853378

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SN - 1071-412X

VL - 10

SP - 514

EP - 519

JO - Clinical and Diagnostic Laboratory Immunology

JF - Clinical and Diagnostic Laboratory Immunology

IS - 4

ER -

Enzyme-linked immunosorbent assay for quantitation of human antibodies to pneumococcal polysaccharides

Abstract

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