Effects of growth at low water activity on the thermal tolerance of Staphylococcus aureus

J. R. Shebuski, O. Vilhelmsson, K. J. Miller*

*Corresponding author for this work

    Research output: Contribution to journalArticlepeer-review

    16 Citations (Scopus)

    Abstract

    Staphylococcus aureus is the most osmotolerant foodborne pathogen, and outbreaks of staphylococcal food poisoning are often linked to foods of reduced water activity (a(w)) values. While it is generally known that the thermal tolerance of micro-organisms increases as the a(w) of the heating menstruum is decreased, surprisingly little research has examined the influence of growth medium a(w) on microbial thermal tolerance. In the present study, we show that growth of S. aureus at an a(w) value of 0.94 leads to the development of dramatically enhanced thermal tolerance (i.e., less than 1 log reduction after heating for 20 min at 60°C). We further show that the identity of the accumulated compatible solute within cells grown at low a(w) can also influence the overall level of thermal tolerance of S. aureus. Finally, we provide evidence that the synthesis of general stress and/or osmotic stress proteins is required for the development of enhanced thermal tolerance of S. aureus at low a(w).

    Original languageEnglish
    Pages (from-to)1277-1281
    Number of pages5
    JournalJournal of Food Protection
    Volume63
    Issue number9
    DOIs
    Publication statusPublished - 2000

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