Effect of calcium chloride treatment on hybridoma cell viability and growth

Gyun Min Lee*, Bong Kwan Han, Jung Hoe Kim, Bernhard O. Palsson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

In order to minimize hybridoma cell damage during calcium alginate entrapment, the effect of calcium chloride treatment on hybridoma cell viability and growth was studied in terms of calcium chloride concentration and treatment time. The cell viability as measured by trypan blue exclusion did not decrease rapidly during the first hour of calcium chloride treatment regardless of calcium chloride concentrations used (1.3 and 1.5%). However, 1.3% calcium chloride solution appeared to be more detrimental to the cells than 1.5% calcium chloride solution. The cells in 1.3% calcium chloride solution lost their viability faster than the cells in 1.5% calcium chloride solution. In addition, when the cells treated with calcium chloride were inoculated into spinner flasks containing IMDM with 10% fetal calf serum, the cells treated with 1.3% calcium chloride solution showed a longer lag phase than the cells treated with 1.5% calcium chloride solution.

Original languageEnglish
Pages (from-to)891-896
Number of pages6
JournalBiotechnology Letters
Volume14
Issue number10
DOIs
Publication statusPublished - Oct 1992

Fingerprint

Dive into the research topics of 'Effect of calcium chloride treatment on hybridoma cell viability and growth'. Together they form a unique fingerprint.

Cite this