Distribution of CA 125 in embryonic tissues and adult derivatives of the fetal periderm

Hildur Hardardottir; Tim H. Parmley; J. Gerald Quirk; M. Melinda Sanders; Frank C. Miller; Timothy J. O'Brien

doi:10.1016/0002-9378(90)90775-3

Distribution of CA 125 in embryonic tissues and adult derivatives of the fetal periderm

Hildur Hardardottir, Tim H. Parmley, J. Gerald Quirk, M. Melinda Sanders, Frank C. Miller, Timothy J. O'Brien^*

^*Corresponding author for this work

Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

54 Citations (Scopus)

Abstract

New murine monoclonal antibodies to a partially purified CA 125 antigen were developed and identified as M 2 and M 11. With immunohistochemical techniques, these new antibodies and OC 125 antibody were used to search for CA 125 in embryonic tissues and adult apocrine sweat glands and mammary glands. The embryonic skin, the periderm, expressed CA 125 antigen and its adult derivatives, the mammary glands and apocrine sweat glands, expressed CA 125 while in the active state of secretion. In a 6-week-old formalin-fixed and paraffin-embedded ectopic embryo specimen, antibodies M 2 and M 11 recognized CA 125 in the periderm, the notochord, the myocardium, the pericardium, the gastroenteric tract, enteric duct remnants in the umbilical cord (vitelline and allantoic ducts), the mesonephric duct, and the amnion. OC 125 staining of these formalin-fixed specimens was either very faint or absent. In a formalin-fixed and paraffin-embedded specimen of apocrine sweat glands from the axilla, antibodies M 2 and M 11 detected CA 125 antigen intracellularly in the secretory cells. Again no staining was observed with OC 125 antibody. In a frozen and acetone-fixed specimen of lactating mammary glands, antibodies M 2 and OC 125 detected CA 125 antigen intraductally. Colostrum and milk collected from 25 mothers at various stages post partum had mean CA 125 levels of 34,213 U/ml in colostrum 1469 U/ml at 3 to 7 days, and 105 U/ml at 5 to 26 weeks.

Original language	English
Pages (from-to)	1925-1931
Number of pages	7
Journal	American Journal of Obstetrics and Gynecology
Volume	163
Issue number	6 PART 1
DOIs	https://doi.org/10.1016/0002-9378(90)90775-3
Publication status	Published - Dec 1990

Bibliographical note

Funding Information:
From the Departments of Obstetrics and Gynecology" and Pathology,' University of Arkansas for Medical Sciences, Supported by National Institutes of Health Grant No, ROICA40406, Presented at the Thirty-seventh Annual Meeting of the Society for G,~necologic Investigation, St, Louis, Missouri, March 21-24, 1990, Reprint requests: Timothy J. O'Brien, PhD, University of Arkansas for Medical Sciences, Department of Obstetrics and Gynecology, 4301 W. Markham, Slot 518, Little Rock, AR 72205, 6/6/24776

Other keywords

apocrine glands
breast milk
CA 125
periderm

Access to Document

10.1016/0002-9378(90)90775-3

Cite this

@article{a9d1ad06180d456296225fa9206a6a34,

title = "Distribution of CA 125 in embryonic tissues and adult derivatives of the fetal periderm",

abstract = "New murine monoclonal antibodies to a partially purified CA 125 antigen were developed and identified as M 2 and M 11. With immunohistochemical techniques, these new antibodies and OC 125 antibody were used to search for CA 125 in embryonic tissues and adult apocrine sweat glands and mammary glands. The embryonic skin, the periderm, expressed CA 125 antigen and its adult derivatives, the mammary glands and apocrine sweat glands, expressed CA 125 while in the active state of secretion. In a 6-week-old formalin-fixed and paraffin-embedded ectopic embryo specimen, antibodies M 2 and M 11 recognized CA 125 in the periderm, the notochord, the myocardium, the pericardium, the gastroenteric tract, enteric duct remnants in the umbilical cord (vitelline and allantoic ducts), the mesonephric duct, and the amnion. OC 125 staining of these formalin-fixed specimens was either very faint or absent. In a formalin-fixed and paraffin-embedded specimen of apocrine sweat glands from the axilla, antibodies M 2 and M 11 detected CA 125 antigen intracellularly in the secretory cells. Again no staining was observed with OC 125 antibody. In a frozen and acetone-fixed specimen of lactating mammary glands, antibodies M 2 and OC 125 detected CA 125 antigen intraductally. Colostrum and milk collected from 25 mothers at various stages post partum had mean CA 125 levels of 34,213 U/ml in colostrum 1469 U/ml at 3 to 7 days, and 105 U/ml at 5 to 26 weeks.",

keywords = "apocrine glands, breast milk, CA 125, periderm",

author = "Hildur Hardardottir and Parmley, {Tim H.} and Quirk, {J. Gerald} and Sanders, {M. Melinda} and Miller, {Frank C.} and O'Brien, {Timothy J.}",

note = "Funding Information: From the Departments of Obstetrics and Gynecology{"} and Pathology,' University of Arkansas for Medical Sciences, Supported by National Institutes of Health Grant No, ROICA40406, Presented at the Thirty-seventh Annual Meeting of the Society for G,~necologic Investigation, St, Louis, Missouri, March 21-24, 1990, Reprint requests: Timothy J. O'Brien, PhD, University of Arkansas for Medical Sciences, Department of Obstetrics and Gynecology, 4301 W. Markham, Slot 518, Little Rock, AR 72205, 6/6/24776",

year = "1990",

month = dec,

doi = "10.1016/0002-9378(90)90775-3",

language = "English",

volume = "163",

pages = "1925--1931",

journal = "American Journal of Obstetrics and Gynecology",

issn = "0002-9378",

publisher = "Mosby Inc.",

number = "6 PART 1",

}

TY - JOUR

T1 - Distribution of CA 125 in embryonic tissues and adult derivatives of the fetal periderm

AU - Hardardottir, Hildur

AU - Parmley, Tim H.

AU - Quirk, J. Gerald

AU - Sanders, M. Melinda

AU - Miller, Frank C.

AU - O'Brien, Timothy J.

N1 - Funding Information: From the Departments of Obstetrics and Gynecology" and Pathology,' University of Arkansas for Medical Sciences, Supported by National Institutes of Health Grant No, ROICA40406, Presented at the Thirty-seventh Annual Meeting of the Society for G,~necologic Investigation, St, Louis, Missouri, March 21-24, 1990, Reprint requests: Timothy J. O'Brien, PhD, University of Arkansas for Medical Sciences, Department of Obstetrics and Gynecology, 4301 W. Markham, Slot 518, Little Rock, AR 72205, 6/6/24776

PY - 1990/12

Y1 - 1990/12

N2 - New murine monoclonal antibodies to a partially purified CA 125 antigen were developed and identified as M 2 and M 11. With immunohistochemical techniques, these new antibodies and OC 125 antibody were used to search for CA 125 in embryonic tissues and adult apocrine sweat glands and mammary glands. The embryonic skin, the periderm, expressed CA 125 antigen and its adult derivatives, the mammary glands and apocrine sweat glands, expressed CA 125 while in the active state of secretion. In a 6-week-old formalin-fixed and paraffin-embedded ectopic embryo specimen, antibodies M 2 and M 11 recognized CA 125 in the periderm, the notochord, the myocardium, the pericardium, the gastroenteric tract, enteric duct remnants in the umbilical cord (vitelline and allantoic ducts), the mesonephric duct, and the amnion. OC 125 staining of these formalin-fixed specimens was either very faint or absent. In a formalin-fixed and paraffin-embedded specimen of apocrine sweat glands from the axilla, antibodies M 2 and M 11 detected CA 125 antigen intracellularly in the secretory cells. Again no staining was observed with OC 125 antibody. In a frozen and acetone-fixed specimen of lactating mammary glands, antibodies M 2 and OC 125 detected CA 125 antigen intraductally. Colostrum and milk collected from 25 mothers at various stages post partum had mean CA 125 levels of 34,213 U/ml in colostrum 1469 U/ml at 3 to 7 days, and 105 U/ml at 5 to 26 weeks.

AB - New murine monoclonal antibodies to a partially purified CA 125 antigen were developed and identified as M 2 and M 11. With immunohistochemical techniques, these new antibodies and OC 125 antibody were used to search for CA 125 in embryonic tissues and adult apocrine sweat glands and mammary glands. The embryonic skin, the periderm, expressed CA 125 antigen and its adult derivatives, the mammary glands and apocrine sweat glands, expressed CA 125 while in the active state of secretion. In a 6-week-old formalin-fixed and paraffin-embedded ectopic embryo specimen, antibodies M 2 and M 11 recognized CA 125 in the periderm, the notochord, the myocardium, the pericardium, the gastroenteric tract, enteric duct remnants in the umbilical cord (vitelline and allantoic ducts), the mesonephric duct, and the amnion. OC 125 staining of these formalin-fixed specimens was either very faint or absent. In a formalin-fixed and paraffin-embedded specimen of apocrine sweat glands from the axilla, antibodies M 2 and M 11 detected CA 125 antigen intracellularly in the secretory cells. Again no staining was observed with OC 125 antibody. In a frozen and acetone-fixed specimen of lactating mammary glands, antibodies M 2 and OC 125 detected CA 125 antigen intraductally. Colostrum and milk collected from 25 mothers at various stages post partum had mean CA 125 levels of 34,213 U/ml in colostrum 1469 U/ml at 3 to 7 days, and 105 U/ml at 5 to 26 weeks.

KW - apocrine glands

KW - breast milk

KW - CA 125

KW - periderm

UR - http://www.scopus.com/inward/record.url?scp=0025611542&partnerID=8YFLogxK

U2 - 10.1016/0002-9378(90)90775-3

DO - 10.1016/0002-9378(90)90775-3

M3 - Article

C2 - 2256504

AN - SCOPUS:0025611542

SN - 0002-9378

VL - 163

SP - 1925

EP - 1931

JO - American Journal of Obstetrics and Gynecology

JF - American Journal of Obstetrics and Gynecology

IS - 6 PART 1

ER -

Distribution of CA 125 in embryonic tissues and adult derivatives of the fetal periderm

Abstract

Bibliographical note

Other keywords

Access to Document

Fingerprint

Cite this