Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea): Diphyllobothriidea)

Eva Bazsalovicsová; Gabriel Minárik; Katarína Šoltys; Alžbeta Radačovská; Jesper A. Kuhn; Egil Karlsbakk; Karl Skirnisson; Ivica Králová-Hromadová

doi:10.3390/genes11070782

Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea): Diphyllobothriidea)

Eva Bazsalovicsová, Gabriel Minárik, Katarína Šoltys, Alžbeta Radačovská, Jesper A. Kuhn, Egil Karlsbakk, Karl Skirnisson, Ivica Králová-Hromadová

Research output: Contribution to journal › Article › peer-review

Abstract

Dibothriocephalus dendriticus is one of the causative agents of the fish-borne zoonosis diphyllobothriosis. Polymorphic microsatellite markers were originally developed for future genetic studies using microsatellite library screening and next-generation sequencing (NGS). Out of 128 microsatellite candidates selected after NGS analysis, 126 yielded PCR products of the expected size. A declared repetitive motif was confirmed in 92 loci by Sanger sequencing. The level of polymorphism was tested by fragment analysis. Statistical tests for observed and expected heterozygosities and deviations from Hardy–Weinberg equilibrium revealed 14 polymorphic microsatellite loci suitable for studies on the finer genetic structure of global populations of D. dendriticus.

Original language	English
Article number	782
Pages (from-to)	1-8
Number of pages	782
Journal	Genes
Volume	11
Issue number	7
DOIs	https://doi.org/10.3390/genes11070782
Publication status	Published - 12 Jul 2020

Bibliographical note

Funding Information:
Funding: The work was financially supported by the Slovak Research and Development Agency under contract APVV-15-0004 and Slovak Grant Agency VEGA no. 2/0134/17.

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Other keywords

Diphyllobothriosis
Fish-borne zoonosis
Microsatellite library screening
Polymorphic loci
Short tandem repeats
Bandormar
DNA-rannsóknir

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10.3390/genes11070782

https://hdl.handle.net/20.500.11815/2304

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title = "Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea): Diphyllobothriidea)",

abstract = "Dibothriocephalus dendriticus is one of the causative agents of the fish-borne zoonosis diphyllobothriosis. Polymorphic microsatellite markers were originally developed for future genetic studies using microsatellite library screening and next-generation sequencing (NGS). Out of 128 microsatellite candidates selected after NGS analysis, 126 yielded PCR products of the expected size. A declared repetitive motif was confirmed in 92 loci by Sanger sequencing. The level of polymorphism was tested by fragment analysis. Statistical tests for observed and expected heterozygosities and deviations from Hardy–Weinberg equilibrium revealed 14 polymorphic microsatellite loci suitable for studies on the finer genetic structure of global populations of D. dendriticus.",

keywords = "Diphyllobothriosis, Fish-borne zoonosis, Microsatellite library screening, Polymorphic loci, Short tandem repeats, Bandormar, DNA-ranns{\'o}knir, Diphyllobothriosis, Fish-borne zoonosis, Microsatellite library screening, Polymorphic loci, Short tandem repeats, Bandormar, DNA-ranns{\'o}knir",

author = "Eva Bazsalovicsov{\'a} and Gabriel Min{\'a}rik and Katar{\'i}na {\v S}oltys and Al{\v z}beta Rada{\v c}ovsk{\'a} and Kuhn, {Jesper A.} and Egil Karlsbakk and Karl Skirnisson and Ivica Kr{\'a}lov{\'a}-Hromadov{\'a}",

note = "Funding Information: Funding: The work was financially supported by the Slovak Research and Development Agency under contract APVV-15-0004 and Slovak Grant Agency VEGA no. 2/0134/17. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland.",

year = "2020",

month = jul,

day = "12",

doi = "10.3390/genes11070782",

language = "English",

volume = "11",

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AU - Minárik, Gabriel

AU - Šoltys, Katarína

AU - Radačovská, Alžbeta

AU - Kuhn, Jesper A.

AU - Karlsbakk, Egil

AU - Skirnisson, Karl

AU - Králová-Hromadová, Ivica

N1 - Funding Information: Funding: The work was financially supported by the Slovak Research and Development Agency under contract APVV-15-0004 and Slovak Grant Agency VEGA no. 2/0134/17. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2020/7/12

Y1 - 2020/7/12

N2 - Dibothriocephalus dendriticus is one of the causative agents of the fish-borne zoonosis diphyllobothriosis. Polymorphic microsatellite markers were originally developed for future genetic studies using microsatellite library screening and next-generation sequencing (NGS). Out of 128 microsatellite candidates selected after NGS analysis, 126 yielded PCR products of the expected size. A declared repetitive motif was confirmed in 92 loci by Sanger sequencing. The level of polymorphism was tested by fragment analysis. Statistical tests for observed and expected heterozygosities and deviations from Hardy–Weinberg equilibrium revealed 14 polymorphic microsatellite loci suitable for studies on the finer genetic structure of global populations of D. dendriticus.

AB - Dibothriocephalus dendriticus is one of the causative agents of the fish-borne zoonosis diphyllobothriosis. Polymorphic microsatellite markers were originally developed for future genetic studies using microsatellite library screening and next-generation sequencing (NGS). Out of 128 microsatellite candidates selected after NGS analysis, 126 yielded PCR products of the expected size. A declared repetitive motif was confirmed in 92 loci by Sanger sequencing. The level of polymorphism was tested by fragment analysis. Statistical tests for observed and expected heterozygosities and deviations from Hardy–Weinberg equilibrium revealed 14 polymorphic microsatellite loci suitable for studies on the finer genetic structure of global populations of D. dendriticus.

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KW - Fish-borne zoonosis

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KW - Short tandem repeats

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KW - Diphyllobothriosis

KW - Fish-borne zoonosis

KW - Microsatellite library screening

KW - Polymorphic loci

KW - Short tandem repeats

KW - Bandormar

KW - DNA-rannsóknir

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SN - 2073-4425

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ER -

Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea): Diphyllobothriidea)

Abstract

Bibliographical note

Other keywords

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