Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [3H]thymidine incorporation

Magnús Gottfredsson, Helga Erlendsdóttir, Adalsteinn Gudmundsson, Sigurdur Gudmundsson*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


Postantibiotic effects (PAE) and control-related effective regrowth time (CERT) of dicloxacillin, vancomycin, rifampin and gentamicin in Staphylococcus aureus and imipenem, gentamicin, tobramycin, doxycycline and rifampin in Escherichia coli were measured by standard viability counting and [3H]thymidine incorporation. For PAE determination, the two methods correlated well; r2=0.821 for S. aureus and r2=0.939 for E. coli. For viable counts below the detection limits of 105 to 106 log10 CFU/mL, the PAE was overestimated by the [3H]thymidine method. Quantitation of CERT by both methods showed a good correlation, r2=0.867 for S. aureus and r2=0.997 for E. coli. Measuring [3H]thymidine incorporation in bacteria is a novel alternative method for the determination of PAE and CERT.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalClinical Microbiology and Infection
Issue number2
Publication statusPublished - 1 Oct 1996

Bibliographical note

Funding Information:
This work was supported by grants from the Icelandic Science Foundation, The University of Iceland Research Fund and Reykjavík City Hospital Science Fund (S. Gudmundsson). Support from Delta Pharmaceuticals, Hafnarfjordur, Iceland is appreciated. The authors wish to thank Drs Helga à-gmundsdóttir and Ásbjörn Sigfússon for technical advice and laboratory facilities.

Other keywords

  • E. coli
  • Postantibiotic effect
  • S. aureus
  • [H]thymidine incorporation
  • Staphylococcus aureus
  • Escherichia coli
  • Drug Resistance, Multiple, Bacterial


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