Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [3H]thymidine incorporation

Magnús Gottfredsson; Helga Erlendsdóttir; Adalsteinn Gudmundsson; Sigurdur Gudmundsson

doi:10.1111/j.1469-0691.1996.tb00213.x

Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [³H]thymidine incorporation

Magnús Gottfredsson, Helga Erlendsdóttir, Adalsteinn Gudmundsson, Sigurdur Gudmundsson^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Postantibiotic effects (PAE) and control-related effective regrowth time (CERT) of dicloxacillin, vancomycin, rifampin and gentamicin in Staphylococcus aureus and imipenem, gentamicin, tobramycin, doxycycline and rifampin in Escherichia coli were measured by standard viability counting and [³H]thymidine incorporation. For PAE determination, the two methods correlated well; r²=0.821 for S. aureus and r²=0.939 for E. coli. For viable counts below the detection limits of 10⁵ to 10⁶ log₁₀ CFU/mL, the PAE was overestimated by the [³H]thymidine method. Quantitation of CERT by both methods showed a good correlation, r²=0.867 for S. aureus and r²=0.997 for E. coli. Measuring [³H]thymidine incorporation in bacteria is a novel alternative method for the determination of PAE and CERT.

Original language	English
Pages (from-to)	99-104
Number of pages	6
Journal	Clinical Microbiology and Infection
Volume	2
Issue number	2
DOIs	https://doi.org/10.1111/j.1469-0691.1996.tb00213.x
Publication status	Published - 1 Oct 1996

Bibliographical note

Funding Information:
This work was supported by grants from the Icelandic Science Foundation, The University of Iceland Research Fund and Reykjavík City Hospital Science Fund (S. Gudmundsson). Support from Delta Pharmaceuticals, Hafnarfjordur, Iceland is appreciated. The authors wish to thank Drs Helga à-gmundsdóttir and Ásbjörn Sigfússon for technical advice and laboratory facilities.

Other keywords

E. coli
Postantibiotic effect
S. aureus
[H]thymidine incorporation
Staphylococcus aureus
Escherichia coli
Drug Resistance, Multiple, Bacterial

Access to Document

10.1111/j.1469-0691.1996.tb00213.x

http://dx.doi.org/10.1111/j.1469-0691.1996.tb00213.x

Cite this

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title = "Determination of in vitro postantibiotic effects in Staphylococcus aureus and Escherichia coli by [3H]thymidine incorporation",

abstract = "Postantibiotic effects (PAE) and control-related effective regrowth time (CERT) of dicloxacillin, vancomycin, rifampin and gentamicin in Staphylococcus aureus and imipenem, gentamicin, tobramycin, doxycycline and rifampin in Escherichia coli were measured by standard viability counting and [3H]thymidine incorporation. For PAE determination, the two methods correlated well; r2=0.821 for S. aureus and r2=0.939 for E. coli. For viable counts below the detection limits of 105 to 106 log10 CFU/mL, the PAE was overestimated by the [3H]thymidine method. Quantitation of CERT by both methods showed a good correlation, r2=0.867 for S. aureus and r2=0.997 for E. coli. Measuring [3H]thymidine incorporation in bacteria is a novel alternative method for the determination of PAE and CERT.",

keywords = "E. coli, Postantibiotic effect, S. aureus, [H]thymidine incorporation, Staphylococcus aureus, Escherichia coli, Drug Resistance, Multiple, Bacterial, Staphylococcus aureus, Escherichia coli, Drug Resistance, Multiple, Bacterial",

author = "Magn{\'u}s Gottfredsson and Helga Erlendsd{\'o}ttir and Adalsteinn Gudmundsson and Sigurdur Gudmundsson",

note = "Funding Information: This work was supported by grants from the Icelandic Science Foundation, The University of Iceland Research Fund and Reykjav{\'i}k City Hospital Science Fund (S. Gudmundsson). Support from Delta Pharmaceuticals, Hafnarfjordur, Iceland is appreciated. The authors wish to thank Drs Helga {\`a}-gmundsd{\'o}ttir and {\'A}sbj{\"o}rn Sigf{\'u}sson for technical advice and laboratory facilities.",

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AU - Gudmundsson, Adalsteinn

AU - Gudmundsson, Sigurdur

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N2 - Postantibiotic effects (PAE) and control-related effective regrowth time (CERT) of dicloxacillin, vancomycin, rifampin and gentamicin in Staphylococcus aureus and imipenem, gentamicin, tobramycin, doxycycline and rifampin in Escherichia coli were measured by standard viability counting and [3H]thymidine incorporation. For PAE determination, the two methods correlated well; r2=0.821 for S. aureus and r2=0.939 for E. coli. For viable counts below the detection limits of 105 to 106 log10 CFU/mL, the PAE was overestimated by the [3H]thymidine method. Quantitation of CERT by both methods showed a good correlation, r2=0.867 for S. aureus and r2=0.997 for E. coli. Measuring [3H]thymidine incorporation in bacteria is a novel alternative method for the determination of PAE and CERT.

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