TY - JOUR
T1 - Cytokines stimulate lipolysis and decrease lipoprotein lipase activity in cultured fat cells by a prostaglandin independent mechanism
AU - Harðardóttir, Ingibjörg
AU - Doerrler, William
AU - Feingold, Kenneth R.
AU - Grunfeld, Carl
N1 - Funding Information:
This work was supported by grants from the Research Service of the Department of Veterans Affairs and the NIH (DK-40990).
PY - 1992/7/15
Y1 - 1992/7/15
N2 - We previously showed that indomethacin blocked the effect of tumor necrosis factor (TNF) and other cytokines on lipolysis. We now show that TNF stimulates prostaglandin (PG) production, enhances lipolysis and decreases lipoprotein lipase (LPL) activity in 3T3-F442A adipocytes and indomethacin blocks these activities, suggesting that the actions of TNF are mediated by PG's. However, exogenous PGE2 at the levels induced by TNF is not sufficient to affect lipolysis or LPL activity and low doses of indomethacin and flurbiprofen block PG production without affecting TNF's action. Interleukin-1 and interferon-α and γ induce lipolysis and decrease LPL activity but do not stimulate much PG production. These results demonstrate that cytokines enhance lipolysis and decrease LPL activity in 3T3 adipocytes by a PG independent mechanism.
AB - We previously showed that indomethacin blocked the effect of tumor necrosis factor (TNF) and other cytokines on lipolysis. We now show that TNF stimulates prostaglandin (PG) production, enhances lipolysis and decreases lipoprotein lipase (LPL) activity in 3T3-F442A adipocytes and indomethacin blocks these activities, suggesting that the actions of TNF are mediated by PG's. However, exogenous PGE2 at the levels induced by TNF is not sufficient to affect lipolysis or LPL activity and low doses of indomethacin and flurbiprofen block PG production without affecting TNF's action. Interleukin-1 and interferon-α and γ induce lipolysis and decrease LPL activity but do not stimulate much PG production. These results demonstrate that cytokines enhance lipolysis and decrease LPL activity in 3T3 adipocytes by a PG independent mechanism.
UR - http://www.scopus.com/inward/record.url?scp=0026733835&partnerID=8YFLogxK
U2 - 10.1016/S0006-291X(05)80798-3
DO - 10.1016/S0006-291X(05)80798-3
M3 - Article
C2 - 1632769
AN - SCOPUS:0026733835
SN - 0006-291X
VL - 186
SP - 237
EP - 243
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -