Comparative study of pituitary and bacteria-derived human growth hormone by monoclonal antibodies

Ingileif Jónsdóttir*, Hans peter T. Ekre, Peter Perlmann

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

We have established hybridoma lines which secrete mouse monoclonal antibodies (Mabs) to human pituitary growth hormone, hGH. Using indirect competitive ELISA and indirect passive hemagglutination inhibition twelve different Mabs were characterized with regard to cross-reactivity with the hGH-related hormones, human chorionic somatomammotropin, hCS, and human prolactin, hPRL. The reactivity of these Mabs with pituitary hGH was compared to that with either bacterially-produced methionyl-hGH or to that of reduced and S-carboxymethylated hGH, which has an altered conformation. None of the Mabs reacted with hPRL. Four did not react with hCS whereas the others showed varying degree of cross-reactivity with hCS. All Mabs reacted more weakly with reduced and S-carboxymethylated hGH than with the native form of the hormone, which was not seen with conventional rabbit antisera to hGH. Thus in the case of hGH the Mabs are superior to conventional antisera in revealing small conformational differences. However the pituitary and bacterially-derived methionyl-hGH were indistinguishable as determined by the 12 Mabs.

Original languageEnglish
Pages (from-to)871-876
Number of pages6
JournalMolecular Immunology
Volume20
Issue number8
DOIs
Publication statusPublished - Aug 1983

Bibliographical note

Funding Information:
Acknowledgements-Wteh ank Rahul Ghosh for excellent technicala ssistanceT. his work was supportedb y a grant from KabiVitrum, AB.

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